Generation of a human iPSC line (CIBi014-A) from a patient with Parkinson’s disease carrying a novel heterozygotic PARK8 (LRRK2) mutation

Lingfei Li; Xiaoli Si; Jiayin Yang; Meng Lei; Huifang Liu; Jie Ruan; Hanting Fu; Wenxuan Li; Hui Yang; Xiaoguang Lei; Hongfei Sang

Stem Cell Research (Feb 2023)

Generation of a human iPSC line (CIBi014-A) from a patient with Parkinson’s disease carrying a novel heterozygotic PARK8 (LRRK2) mutation

Lingfei Li,
Xiaoli Si,
Jiayin Yang,
Meng Lei,
Huifang Liu,
Jie Ruan,
Hanting Fu,
Wenxuan Li,
Hui Yang,
Xiaoguang Lei,
Hongfei Sang

Affiliations

Lingfei Li: Department of Neurology, Affiliated Hangzhou First People’s Hospital, Zhejiang University School of Medicine, Hangzhou 310000, China
Xiaoli Si: Department of Neurology, the Second Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou 310000, China
Jiayin Yang: Laboratory of iPSC and Disease Models, Cell Inspire Biotechnology Co., Ltd., Shenzhen, 518101, China
Meng Lei: Department of Neurology, The Third People's Hospital of Henan Province, Zhengzhou 450000, China
Huifang Liu: Division of Neurology, Department of Medicine, University of Hong Kong, Hong Kong
Jie Ruan: Department of Clinical Pharmacy, The First Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou 310000, China
Hanting Fu: The 1st School of Medicine, School of Information and Engineering, Wenzhou Medical University, Wenzhou 325000, China
Wenxuan Li: The 1st School of Medicine, School of Information and Engineering, Wenzhou Medical University, Wenzhou 325000, China
Hui Yang: Department of Neurology, Affiliated Hangzhou First People’s Hospital, Zhejiang University School of Medicine, Hangzhou 310000, China
Xiaoguang Lei: Department of Neurology, First Affiliated Hospital of Kunming Medical University, No.1 School of Clinical Medicine, Kunming Medical University, Kunming 650500, China; Corresponding authors.
Hongfei Sang: Department of Neurology, Affiliated Hangzhou First People’s Hospital, Zhejiang University School of Medicine, Hangzhou 310000, China; Corresponding authors.

Journal volume & issue: Vol. 66
p. 102995

Abstract

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Leucine-rich repeat kinase 2 (LRRK2), also known as Dardarin (derived from the Basque word “dardara,” meaning tremor), is a protein kinase encoded by the PARK8 gene. This protein is mainly distributed in the cytoplasm; however, it is also present in the mitochondrial outer membrane. Mutations in the LRRK2 protein cause Parkinson's disease (PD). We generated a human induced pluripotent stem cell (iPSC) line (CIBi014-A) expressing pluripotency markers. This cell line exhibited a normal karyotype and could be differentiated into three germ layers in vitro. This line will be valuable for investigating the mechanism underlying PD and identifying potential therapeutic targets.

Published in Stem Cell Research

ISSN: 1873-5061 (Print); 1876-7753 (Online)
Publisher: Elsevier
Country of publisher: Netherlands
LCC subjects: Science: Biology (General)
Website: https://www.journals.elsevier.com/stem-cell-research

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