Frontiers in Plant Science (Oct 2022)
PpZAT5 suppresses the expression of a B-box gene PpBBX18 to inhibit anthocyanin biosynthesis in the fruit peel of red pear
- Lu Zhang,
- Lu Zhang,
- Lu Zhang,
- Ruiyan Tao,
- Ruiyan Tao,
- Ruiyan Tao,
- Simai Wang,
- Simai Wang,
- Simai Wang,
- Yuhao Gao,
- Yuhao Gao,
- Yuhao Gao,
- Lu Wang,
- Lu Wang,
- Lu Wang,
- Shulin Yang,
- Shulin Yang,
- Shulin Yang,
- Xiao Zhang,
- Xiao Zhang,
- Xiao Zhang,
- Wenjie Yu,
- Wenjie Yu,
- Wenjie Yu,
- Xinyue Wu,
- Xinyue Wu,
- Xinyue Wu,
- Kunfeng Li,
- Junbei Ni,
- Junbei Ni,
- Junbei Ni,
- Yuanwen Teng,
- Yuanwen Teng,
- Yuanwen Teng,
- Songling Bai,
- Songling Bai,
- Songling Bai
Affiliations
- Lu Zhang
- Department of Horticulture, Zhejiang University, Hangzhou, China
- Lu Zhang
- Zhejiang Provincial Key Laboratory of Integrative Biology of Horticultural Plants, Zhejiang University, Hangzhou, China
- Lu Zhang
- The Key Laboratory of Horticultural Plant Growth, Development and Quality Improvement, Ministry of Agriculture of China, Hangzhou, China
- Ruiyan Tao
- Department of Horticulture, Zhejiang University, Hangzhou, China
- Ruiyan Tao
- Zhejiang Provincial Key Laboratory of Integrative Biology of Horticultural Plants, Zhejiang University, Hangzhou, China
- Ruiyan Tao
- The Key Laboratory of Horticultural Plant Growth, Development and Quality Improvement, Ministry of Agriculture of China, Hangzhou, China
- Simai Wang
- Department of Horticulture, Zhejiang University, Hangzhou, China
- Simai Wang
- Zhejiang Provincial Key Laboratory of Integrative Biology of Horticultural Plants, Zhejiang University, Hangzhou, China
- Simai Wang
- The Key Laboratory of Horticultural Plant Growth, Development and Quality Improvement, Ministry of Agriculture of China, Hangzhou, China
- Yuhao Gao
- Department of Horticulture, Zhejiang University, Hangzhou, China
- Yuhao Gao
- Zhejiang Provincial Key Laboratory of Integrative Biology of Horticultural Plants, Zhejiang University, Hangzhou, China
- Yuhao Gao
- The Key Laboratory of Horticultural Plant Growth, Development and Quality Improvement, Ministry of Agriculture of China, Hangzhou, China
- Lu Wang
- Department of Horticulture, Zhejiang University, Hangzhou, China
- Lu Wang
- Zhejiang Provincial Key Laboratory of Integrative Biology of Horticultural Plants, Zhejiang University, Hangzhou, China
- Lu Wang
- The Key Laboratory of Horticultural Plant Growth, Development and Quality Improvement, Ministry of Agriculture of China, Hangzhou, China
- Shulin Yang
- Department of Horticulture, Zhejiang University, Hangzhou, China
- Shulin Yang
- Zhejiang Provincial Key Laboratory of Integrative Biology of Horticultural Plants, Zhejiang University, Hangzhou, China
- Shulin Yang
- The Key Laboratory of Horticultural Plant Growth, Development and Quality Improvement, Ministry of Agriculture of China, Hangzhou, China
- Xiao Zhang
- Department of Horticulture, Zhejiang University, Hangzhou, China
- Xiao Zhang
- Zhejiang Provincial Key Laboratory of Integrative Biology of Horticultural Plants, Zhejiang University, Hangzhou, China
- Xiao Zhang
- The Key Laboratory of Horticultural Plant Growth, Development and Quality Improvement, Ministry of Agriculture of China, Hangzhou, China
- Wenjie Yu
- Department of Horticulture, Zhejiang University, Hangzhou, China
- Wenjie Yu
- Zhejiang Provincial Key Laboratory of Integrative Biology of Horticultural Plants, Zhejiang University, Hangzhou, China
- Wenjie Yu
- The Key Laboratory of Horticultural Plant Growth, Development and Quality Improvement, Ministry of Agriculture of China, Hangzhou, China
- Xinyue Wu
- Department of Horticulture, Zhejiang University, Hangzhou, China
- Xinyue Wu
- Zhejiang Provincial Key Laboratory of Integrative Biology of Horticultural Plants, Zhejiang University, Hangzhou, China
- Xinyue Wu
- The Key Laboratory of Horticultural Plant Growth, Development and Quality Improvement, Ministry of Agriculture of China, Hangzhou, China
- Kunfeng Li
- Agricultural Experiment Station, Zhejiang University, Hangzhou, China
- Junbei Ni
- Department of Horticulture, Zhejiang University, Hangzhou, China
- Junbei Ni
- Zhejiang Provincial Key Laboratory of Integrative Biology of Horticultural Plants, Zhejiang University, Hangzhou, China
- Junbei Ni
- The Key Laboratory of Horticultural Plant Growth, Development and Quality Improvement, Ministry of Agriculture of China, Hangzhou, China
- Yuanwen Teng
- Department of Horticulture, Zhejiang University, Hangzhou, China
- Yuanwen Teng
- Zhejiang Provincial Key Laboratory of Integrative Biology of Horticultural Plants, Zhejiang University, Hangzhou, China
- Yuanwen Teng
- The Key Laboratory of Horticultural Plant Growth, Development and Quality Improvement, Ministry of Agriculture of China, Hangzhou, China
- Songling Bai
- Department of Horticulture, Zhejiang University, Hangzhou, China
- Songling Bai
- Zhejiang Provincial Key Laboratory of Integrative Biology of Horticultural Plants, Zhejiang University, Hangzhou, China
- Songling Bai
- The Key Laboratory of Horticultural Plant Growth, Development and Quality Improvement, Ministry of Agriculture of China, Hangzhou, China
- DOI
- https://doi.org/10.3389/fpls.2022.1022034
- Journal volume & issue
-
Vol. 13
Abstract
BBX (B-box) proteins play a vital role in light-induced anthocyanin biosynthesis. PpBBX18 was an indispensable regulator for the induction of anthocyanin biosynthesis in the peel of red pear fruit (Pyrus pyrifolia Nakai.). However, the upstream regulation of BBX genes has not been well characterized. In this study, PpZAT5, a cysteine2/histidine2-type transcription factor, was discovered as the upstream negative regulator of PpBBX18. The results showed that PpZAT5 functions as a transcriptional repressor and directly binds to the CAAT motif of PpBBX18 and inhibits its expression. PpZAT5 expression was inhibited by light, which is converse to the expression pattern of anthocyanin-related structural genes. In addition, less anthocyanin accumulated in the PpZAT5-overexpressing pear calli than in the wild-type pear calli; on the contrary, more anthocyanin accumulated in PpZAT5-RNAi pear calli. Moreover, the crucial genes involved in light-induced anthocyanin biosynthesis were markedly down-regulated in the transcriptome of PpZAT5 overexpression pear calli compared to wild-type. In conclusion, our study indicates that PpBBX18 is negatively regulated by a C2H2-type transcriptional repressor, PpZAT5, which reduces anthocyanin content in pear. The present results demonstrate an upstream molecular mechanism of PpBBX18 and provide insights into light-induced anthocyanin biosynthesis.
Keywords
