Frontiers in Oncology (Feb 2022)

Single-Cell Transcriptome Comparison of Bladder Cancer Reveals Its Ecosystem

  • Yongxiang Luo,
  • Yongxiang Luo,
  • Tao Tao,
  • Tao Tao,
  • Ran Tao,
  • Guixiao Huang,
  • Song Wu,
  • Song Wu,
  • Song Wu

DOI
https://doi.org/10.3389/fonc.2022.818147
Journal volume & issue
Vol. 12

Abstract

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Bladder carcinoma (BLCA) is a highly heterogeneous disease, and the underlying biological behavior is still poorly understood. Here, single-cell RNA sequencing was performed on four clinical samples of different grades from three patients, and 26,792 cell transcriptomes were obtained revealing different tumor ecosystems. We found that N-glycan biosynthesis pathway was activated in high-grade tumor, but TNF-related pathway was activated in cystitis glandularis. The tumor microenvironment (TME) of different samples showed great heterogeneity. Notably, cystitis glandularis was dominated by T cells, low-grade and high-grade tumors by macrophages, while TME in patient with high-grade relapse by stromal cells. Our research provides single-cell transcriptome profiles of cystitis glandularis and BLCA in different clinical states, and the biological program revealed by single-cell data can be used as biomarkers related to clinical prognosis in independent cohorts.

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