Nature Communications (Jul 2017)
Accumulating the hydride state in the catalytic cycle of [FeFe]-hydrogenases
Abstract
Of the four intermediates in the catalytic cycle of [FeFe]-hydrogenases, the hydride state still has eluded characterization. Here, the authors shift the reaction equilibrium for three hydrogenases and enrich the hydride-bound species, characterizing them using a real-time IR spectroscopic technique.