Nature Communications (Jul 2017)

Accumulating the hydride state in the catalytic cycle of [FeFe]-hydrogenases

  • Martin Winkler,
  • Moritz Senger,
  • Jifu Duan,
  • Julian Esselborn,
  • Florian Wittkamp,
  • Eckhard Hofmann,
  • Ulf-Peter Apfel,
  • Sven Timo Stripp,
  • Thomas Happe

DOI
https://doi.org/10.1038/ncomms16115
Journal volume & issue
Vol. 8, no. 1
pp. 1 – 7

Abstract

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Of the four intermediates in the catalytic cycle of [FeFe]-hydrogenases, the hydride state still has eluded characterization. Here, the authors shift the reaction equilibrium for three hydrogenases and enrich the hydride-bound species, characterizing them using a real-time IR spectroscopic technique.