Scientific Papers Animal Science and Biotechnologies (Oct 2023)
GLUTATHIONE DYNAMICS IN THE SECOND GENERATION YOUNG RATS BLOOD AS A CONSEQUENCE OF FEMALE EXPOSURE TO Cr(VI) INTOXICATION DURING GESTATION
Abstract
Chromium compounds are found in the environment, due to erosion of chromium containing rocks and can be distributed by volcanic eruptions in food, water. Metals being non-biodegradable persist in the environment for a long period and cause serious ecotoxicological problems. Chromium, which exists in nature mostly in the trivalent form (Cr+3), is essential for activating certain enzymes and for stabilizing proteins and nucleic acids. We have studied the influence of the glutathione dynamics in the second generation rats blood, as a consequence of females chromium (VI) intoxication during the gestation. This study was carried out on 7 Wistar adult female rats, control group (C), 21 adult Wistar female rats, devided in three experimental groups (E) and theire young rats. The rats were feet, durind the gestation, with 25ppm (LOAEL), 50ppm and 75ppm potassium dichromate, ad libitum, in drinking water. The control batch received tap water. Reduced glutathione (GSH) was measured quantitatively after the wean using a Perkin-Elmer spectrophotometer, through Beutler et al. method, at 412nm. The study reports also the depletion of young rats blood GSH.