Comparative Studies on Polyphenolic Composition, Antioxidant and Antimicrobial Activities of Schisandra chinensis Leaves and Fruits
Andrei Mocan,
Gianina Crișan,
Laurian Vlase,
Ovidiu Crișan,
Dan Cristian Vodnar,
Oana Raita,
Ana-Maria Gheldiu,
Anca Toiu,
Radu Oprean,
Ioan Tilea
Affiliations
Andrei Mocan
Department of Pharmaceutical Botany, Iuliu Hațieganu University of Medicine and Pharmacy, 12 I. Creangă Street, Cluj-Napoca 400010, Romania
Gianina Crișan
Department of Pharmaceutical Botany, Iuliu Hațieganu University of Medicine and Pharmacy, 12 I. Creangă Street, Cluj-Napoca 400010, Romania
Laurian Vlase
Department of Pharmaceutical Technology and Biopharmaceutics, Iuliu Hațieganu University of Medicine and Pharmacy, 12 I. Creangă Street, Cluj-Napoca 400010, Romania
Ovidiu Crișan
Department of Organic Chemistry, Iuliu Hațieganu University of Medicine and Pharmacy, 12 I. Creangă Street, Cluj- Napoca 400010, Romania
Dan Cristian Vodnar
Department of Food Science and Technology, University of Agricultural Sciences and Veterinary Medicine, 3-5 Manăştur Street, Cluj-Napoca 400372, Romania
Oana Raita
Department of Physics of Nanostructured Materials, National Institute for Research and Development of Isotopic and Molecular Technologies 65-103, Donath Street, Cluj-Napoca 400010, Romania
Ana-Maria Gheldiu
Department of Pharmaceutical Technology and Biopharmaceutics, Iuliu Hațieganu University of Medicine and Pharmacy, 12 I. Creangă Street, Cluj-Napoca 400010, Romania
Anca Toiu
Department of Pharmacognosy, Iuliu Hațieganu University of Medicine and Pharmacy, 12 I. Creangă Street, Cluj-Napoca 400010, Romania
Radu Oprean
Department of Analytical Chemistry and Instrumental Analysis, Iuliu Hațieganu University of Medicine and Pharmacy, 4 L. Pasteur Street, Cluj-Napoca 400010, Romania
Ioan Tilea
Family Medicine, Department M3 Clinical Sciences Internal Medicine, University of Medicine and Pharmacy, 38 G. Marinescu Street, Târgu Mures 540139, Romania
The aim of this paper was to evaluate the antioxidant and antimicrobial activities and the polyphenolic content of Schisandra chinensis (Turcz.) Baill. leaves and fruits. The leaves are an important source of flavonoids (35.10 ± 1.23 mg RE/g plant material). Qualitative and quantitative analyses of the polyphenolic compounds were achieved using a HPLC-UV-MS method. The main flavonoid from the leaves was isoquercitrin (2486.18 ± 5.72 μg/g plant material), followed by quercitrin (1645.14 ± 2.12 μg/g plant material). Regarding the fruit composition, the dominant compound there was rutin (13.02 ± 0.21 μg/g plant material), but comparing with the leaves, fruits can be considered a poor source of phenolic compounds. The antioxidant activity was evaluated by DPPH, TEAC, hemoglobin ascorbate peroxidase activity inhibition (HAPX), inhibition of lipid peroxidation catalyzed by cytochrome c and EPR spectroscopic assays, revealing a better antioxidant activity for the S. chinensis leaves extract. In the antimicrobial assay, S. chinensis leaves extract showed efficient activities against the targeted bacteria, being more active than the fruits extract. The results suggest the leaves of S. chinensis as a valuable source of antioxidant compounds with significant antioxidant activity.
