Genetic and QTL analysis of flower color and pigments in small-flowered chrysanthemum based on high-density genetic map

Xuebin Song; Yuankai Tian; Kang Gao; Junzhuo Li; Yanfei Li; Jiaying Wang; Chengyan Deng; Fan Zhang; Deyuan Kong; Guangxun Fan; Silan Dai

doi:10.48130/OPR-2023-0017

Ornamental Plant Research (Jan 2023)

Genetic and QTL analysis of flower color and pigments in small-flowered chrysanthemum based on high-density genetic map

Xuebin Song,
Yuankai Tian,
Kang Gao,
Junzhuo Li,
Yanfei Li,
Jiaying Wang,
Chengyan Deng,
Fan Zhang,
Deyuan Kong,
Guangxun Fan,
Silan Dai

Affiliations

Xuebin Song: National Engineering Research Center for Floriculture, Beijing Key Laboratory of Ornamental Plants Germplasm Innovation & Molecular Breeding, Beijing Laboratory of Urban and Rural Ecological Environment, School of Landscape Architecture, Beijing Forestry University, Beijing 100083, China
Yuankai Tian: National Engineering Research Center for Floriculture, Beijing Key Laboratory of Ornamental Plants Germplasm Innovation & Molecular Breeding, Beijing Laboratory of Urban and Rural Ecological Environment, School of Landscape Architecture, Beijing Forestry University, Beijing 100083, China
Kang Gao: National Engineering Research Center for Floriculture, Beijing Key Laboratory of Ornamental Plants Germplasm Innovation & Molecular Breeding, Beijing Laboratory of Urban and Rural Ecological Environment, School of Landscape Architecture, Beijing Forestry University, Beijing 100083, China
Junzhuo Li: National Engineering Research Center for Floriculture, Beijing Key Laboratory of Ornamental Plants Germplasm Innovation & Molecular Breeding, Beijing Laboratory of Urban and Rural Ecological Environment, School of Landscape Architecture, Beijing Forestry University, Beijing 100083, China
Yanfei Li: National Engineering Research Center for Floriculture, Beijing Key Laboratory of Ornamental Plants Germplasm Innovation & Molecular Breeding, Beijing Laboratory of Urban and Rural Ecological Environment, School of Landscape Architecture, Beijing Forestry University, Beijing 100083, China
Jiaying Wang: National Engineering Research Center for Floriculture, Beijing Key Laboratory of Ornamental Plants Germplasm Innovation & Molecular Breeding, Beijing Laboratory of Urban and Rural Ecological Environment, School of Landscape Architecture, Beijing Forestry University, Beijing 100083, China
Chengyan Deng: National Engineering Research Center for Floriculture, Beijing Key Laboratory of Ornamental Plants Germplasm Innovation & Molecular Breeding, Beijing Laboratory of Urban and Rural Ecological Environment, School of Landscape Architecture, Beijing Forestry University, Beijing 100083, China
Fan Zhang: National Engineering Research Center for Floriculture, Beijing Key Laboratory of Ornamental Plants Germplasm Innovation & Molecular Breeding, Beijing Laboratory of Urban and Rural Ecological Environment, School of Landscape Architecture, Beijing Forestry University, Beijing 100083, China
Deyuan Kong: National Engineering Research Center for Floriculture, Beijing Key Laboratory of Ornamental Plants Germplasm Innovation & Molecular Breeding, Beijing Laboratory of Urban and Rural Ecological Environment, School of Landscape Architecture, Beijing Forestry University, Beijing 100083, China
Guangxun Fan: National Engineering Research Center for Floriculture, Beijing Key Laboratory of Ornamental Plants Germplasm Innovation & Molecular Breeding, Beijing Laboratory of Urban and Rural Ecological Environment, School of Landscape Architecture, Beijing Forestry University, Beijing 100083, China
Silan Dai: National Engineering Research Center for Floriculture, Beijing Key Laboratory of Ornamental Plants Germplasm Innovation & Molecular Breeding, Beijing Laboratory of Urban and Rural Ecological Environment, School of Landscape Architecture, Beijing Forestry University, Beijing 100083, China

DOI: https://doi.org/10.48130/OPR-2023-0017
Journal volume & issue: Vol. 3, no. 1
pp. 1 – 11

Abstract

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Flower color is an important trait for ornamental plants and is determined by pigments. Variations in the flower color of chrysanthemum are abundant, which is an important feature related to its ornamental and commercial value. However, few reports have elucidated the inheritance of the flower color and pigmentation of chrysanthemum. In this study, an F1 hybrid population, including 319 hybrids of small-flowered chrysanthemum cultivars with different flower color, was constructed. The genetic variation rules for some color and pigment-related traits were discussed based on major gene and polygene mixed inheritance analyses. Both the total anthocyanin and carotenoid contents were controlled by two pairs of additive dominant major genes and the heritability of the major genes was 70.44% and 86.03%, respectively. Based on the high-density genetic map constructed for the hybrid population, QTL analysis for the above traits was carried out using the interval mapping method. A total of 25 related QTLs and 517 closely linked markers were detected, including three QTLs controlling the total anthocyanin content and two QTLs controlling the total carotenoid content, all of which were major QTLs. Furthermore, 17 unigenes related to pigments were identified via BLAST searches with Chrysanthemum nankingense genome. For such genes, the collinearity was observed in four chromosome level genomes, including three genomes of the genus Chrysanthemum and the first genome of cultivated chrysanthemum. The results lay a foundation for in-depth exploration of flower color and pigmentation in chrysanthemum and provide a reference for future research in other ornamental plants.

Published in Ornamental Plant Research

ISSN: 2769-2094 (Online)
Publisher: Maximum Academic Press
Country of publisher: China
LCC subjects: Science: Botany: Plant ecology; Technology: Environmental technology. Sanitary engineering: Environmental effects of industries and plants
Website: https://www.maxapress.com/opr

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