Nature Communications (Apr 2021)
Design of multi-scale protein complexes by hierarchical building block fusion
- Yang Hsia,
- Rubul Mout,
- William Sheffler,
- Natasha I. Edman,
- Ivan Vulovic,
- Young-Jun Park,
- Rachel L. Redler,
- Matthew J. Bick,
- Asim K. Bera,
- Alexis Courbet,
- Alex Kang,
- T. J. Brunette,
- Una Nattermann,
- Evelyn Tsai,
- Ayesha Saleem,
- Cameron M. Chow,
- Damian Ekiert,
- Gira Bhabha,
- David Veesler,
- David Baker
Affiliations
- Yang Hsia
- Department of Biochemistry, University of Washington
- Rubul Mout
- Department of Biochemistry, University of Washington
- William Sheffler
- Department of Biochemistry, University of Washington
- Natasha I. Edman
- Department of Biochemistry, University of Washington
- Ivan Vulovic
- Department of Biochemistry, University of Washington
- Young-Jun Park
- Department of Biochemistry, University of Washington
- Rachel L. Redler
- Department of Cell Biology and Skirball Institute of Biomolecular Medicine, New York University School of Medicine
- Matthew J. Bick
- Department of Biochemistry, University of Washington
- Asim K. Bera
- Department of Biochemistry, University of Washington
- Alexis Courbet
- Department of Biochemistry, University of Washington
- Alex Kang
- Department of Biochemistry, University of Washington
- T. J. Brunette
- Department of Biochemistry, University of Washington
- Una Nattermann
- Department of Biochemistry, University of Washington
- Evelyn Tsai
- Department of Biochemistry, University of Washington
- Ayesha Saleem
- Department of Biochemistry, University of Washington
- Cameron M. Chow
- Department of Biochemistry, University of Washington
- Damian Ekiert
- Department of Cell Biology and Skirball Institute of Biomolecular Medicine, New York University School of Medicine
- Gira Bhabha
- Department of Cell Biology and Skirball Institute of Biomolecular Medicine, New York University School of Medicine
- David Veesler
- Department of Biochemistry, University of Washington
- David Baker
- Department of Biochemistry, University of Washington
- DOI
- https://doi.org/10.1038/s41467-021-22276-z
- Journal volume & issue
-
Vol. 12,
no. 1
pp. 1 – 10
Abstract
De novo design of self-assembling protein nanostructures and materials is of significant interest, however design of complex, multi-component assemblies is challenging. Here, the authors present a stepwise hierarchical approach to build such assemblies using helical repeat and helical bundle proteins as building blocks, and provide an in-depth structural characterization of the resulting assemblies.
