Успехи молекулярной онкологии (Dec 2022)

Diagnostic and prognostic value of long non-coding RNA PROX1‑AS1 and miR-647 expression in gastric cancer

  • E. A. Vetchinkina,
  • A. I. Kalinkin,
  • E. B. Kuznetsova,
  • A. E. Kiseleva,
  • E. A. Alekseeva,
  • M. V. Nemtsova,
  • I. V. Bure

DOI
https://doi.org/10.17650/2313-805X-2022-9-4-50-60
Journal volume & issue
Vol. 9, no. 4

Abstract

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Introduction. Gastric cancer remains one of the most common cancers and has a high mortality rate worldwide. Epigenetic alternations of non-coding RNAs (ncRNAs), including microRNAs and long ncRNAs can contribute to its pathogenesis and progression, and could be potent diagnostic and prognostic biomarkers.Aim. Estimation of PROX1‑AS1 and miR-647 expression in gastric cancer and investigation of its clinical significance. Materials and methods. Tumor and adjacent normal tissues (n = 62), and sectional normal tissue samples (n = 5) were included in the study. The expression of the ncRNAs was quantified by reverse transcription-polymerase chain reaction assay.Results. We have reviled the significant difference in the PROX1‑AS1 expression in tumor (p = 0.002) and non-tumor tissues (p <0.001) obtained from gastric cancer patients in comparison with sectional gastric tissues without pathology. Pearson correlation analysis confirmed a negative correlation between PROX1‑AS1 and miR-647 in gastric cancer both in tumor (р <0,001) and adjacent normal tissues (р <0.001). Besides, expression of PROX1‑AS1 and miR-647 was associated with the size and extent of the primary tumor.Conclusion. The obtained results allow to suggest a potential prognostic value of PROX1‑AS1 and miR-647 in gastric cancer.

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