Evaluation of Total Antioxidant Activity and Oxidative Stress in Seminal Plasma from Dogs Supplemented with Fish Oil and Vitamin E

Abstract

Background: We evaluated the effect of fish oil (FO) and FO in addition to vitamin E (VE) supplementation on totalantioxidant activity of dog seminal plasma, and further assessed oxidative stress. Additionally, we measured the effectof this supplementation on hematological parameters and serum biochemistry.Materials and Methods: In this experimental study, six male dogs were assigned to one of the following three groupsfor a period of 60 days using a replicated 3×3 Latin square design: control (CG), FO (FOG) and FO in addition toVE (FOEG). On days 0 and 60 of the trial, semen and blood samples were obtained. 2,2V-azino-bis (3-ethylbenzothiazoline-6-sulfonate) (ABTS) and ferric reducing antioxidant power (FRAP) assays were used to determine totalantioxidant activity. Oxidative stress was determined by measuring total sulfhydryl group (T-SH).Results: Dogs supplemented with FO alone had a lower total antioxidant activity in seminal plasma (ABTS: -59.86% vs.CG and -57.3% vs. FOEG; and FRAP: -37.3% vs. CG and -40.5% vs. FOEG), and higher oxidative stress (T-SH: +53.0%vs. CG and+60.2% vs. FOEG) compared with the other two groups (p <0.05). Serum triglyceride (TG) concentration decreasedin FOG and FOEG compared with CG, on day 60 (p <0.01).Conclusion: We concluded that total antioxidant activity decreased and oxidative stress increased in seminal plasma ofdogs after FO supplementation for 60 days.

Keywords

• antioxidants
• dog
• fish oil
• oxidative stress
• vitamin e