In Vitro Assessment of Early Bacterial Activity on Micro/Nanostructured Ti6Al4V Surfaces
Benjamin Valdez-Salas,
Ernesto Beltrán-Partida,
Sandra Castillo-Uribe,
Mario Curiel-Álvarez,
Roumen Zlatev,
Margarita Stoytcheva,
Gisela Montero-Alpírez,
Lidia Vargas-Osuna
Affiliations
Benjamin Valdez-Salas
Instituto de Ingeniería, Departamento de Corrosión y Materiales, Universidad Autónoma de Baja California, Blvd. Benito Juárez y Calle de la Normal s/n, Mexicali C.P., 21040 Baja California, Mexico
Ernesto Beltrán-Partida
Instituto de Ingeniería, Departamento de Corrosión y Materiales, Universidad Autónoma de Baja California, Blvd. Benito Juárez y Calle de la Normal s/n, Mexicali C.P., 21040 Baja California, Mexico
Sandra Castillo-Uribe
Instituto de Ingeniería, Departamento de Corrosión y Materiales, Universidad Autónoma de Baja California, Blvd. Benito Juárez y Calle de la Normal s/n, Mexicali C.P., 21040 Baja California, Mexico
Mario Curiel-Álvarez
Instituto de Ingeniería, Departamento de Corrosión y Materiales, Universidad Autónoma de Baja California, Blvd. Benito Juárez y Calle de la Normal s/n, Mexicali C.P., 21040 Baja California, Mexico
Roumen Zlatev
Instituto de Ingeniería, Departamento de Corrosión y Materiales, Universidad Autónoma de Baja California, Blvd. Benito Juárez y Calle de la Normal s/n, Mexicali C.P., 21040 Baja California, Mexico
Margarita Stoytcheva
Instituto de Ingeniería, Departamento de Corrosión y Materiales, Universidad Autónoma de Baja California, Blvd. Benito Juárez y Calle de la Normal s/n, Mexicali C.P., 21040 Baja California, Mexico
Gisela Montero-Alpírez
Instituto de Ingeniería, Departamento de Corrosión y Materiales, Universidad Autónoma de Baja California, Blvd. Benito Juárez y Calle de la Normal s/n, Mexicali C.P., 21040 Baja California, Mexico
Lidia Vargas-Osuna
Facultad de Ingeniería, Universidad Autónoma de Baja California, Blvd. Benito Juárez y Calle de la Normal s/n, Mexicali C.P., 21040 Baja California, Mexico
It is imperative to understand and systematically compare the initial interactions between bacteria genre and surface properties. Thus, we fabricated a flat, anodized with 80 nm TiO2 nanotubes (NTs), and a rough Ti6Al4V surface. The materials were characterized using field-emission scanning electron microscopy (FE-SEM), energy dispersive X-ray spectroscopy (EDX) and atomic force microscopy (AFM). We cultured in vitro Staphylococcus epidermidis (S. epidermidis) and Pseudomonas aeruginosa (P. aeruginosa) to evaluate the bacterial-surface behavior by FE-SEM and viability calculation. In addition, the initial effects of human osteoblasts were tested on the materials. Gram-negative bacteria showed promoted adherence and viability over the flat and rough surface, while NTs displayed opposite activity with altered morphology. Gram-positive bacteria illustrated similar cellular architecture over the surfaces but with promoted surface adhesion bonds on the flat alloy. Rough surfaces supported S. epidermidis viability, whilst NTs exhibited lower vitality. NTs advocated promoted better osteoblast organization with enhanced vitality. Gram-positive bacteria suggested preferred adhesion capability over flat and carbon-rich surfaces. Gram-negative bacteria were strongly disturbed by NTs but largely stimulated by flat and rough materials. Our work proposed that the chemical profile of the material surface and the bacterial cell wall characteristics might play an important role in the bacteria-surface interactions.
