Antigen-Antibody Affinity for Dry Eye Biomarkers by Label Free Biosensing. Comparison with the ELISA Technique
Maríafe Laguna,
Miguel Holgado,
Ana L. Hernandez,
Beatriz Santamaría,
Alvaro Lavín,
Javier Soria,
Tatiana Suarez,
Carlota Bardina,
Mónica Jara,
Francisco J. Sanza,
Rafael Casquel
Affiliations
Maríafe Laguna
Center for Biomedical Technology, Optics, Photonics and Biophotonics Lab, Universidad Politécnica de Madrid. Campus Montegancedo, 28223 Pozuelo de Alarcón, Madrid, Spain
Miguel Holgado
Center for Biomedical Technology, Optics, Photonics and Biophotonics Lab, Universidad Politécnica de Madrid. Campus Montegancedo, 28223 Pozuelo de Alarcón, Madrid, Spain
Ana L. Hernandez
Center for Biomedical Technology, Optics, Photonics and Biophotonics Lab, Universidad Politécnica de Madrid. Campus Montegancedo, 28223 Pozuelo de Alarcón, Madrid, Spain
Beatriz Santamaría
Center for Biomedical Technology, Optics, Photonics and Biophotonics Lab, Universidad Politécnica de Madrid. Campus Montegancedo, 28223 Pozuelo de Alarcón, Madrid, Spain
Alvaro Lavín
Center for Biomedical Technology, Optics, Photonics and Biophotonics Lab, Universidad Politécnica de Madrid. Campus Montegancedo, 28223 Pozuelo de Alarcón, Madrid, Spain
Javier Soria
Bioftalmik. Parque Tecnológico Zamudio Ed. 800 2ª Planta 48160, Bizkaia, Spain
Tatiana Suarez
Bioftalmik. Parque Tecnológico Zamudio Ed. 800 2ª Planta 48160, Bizkaia, Spain
Carlota Bardina
AntibodyBcn, MRB 104 Modul b UAB Campus, 08193 Bellaterra, Barcelona, Spain
Mónica Jara
AntibodyBcn, MRB 104 Modul b UAB Campus, 08193 Bellaterra, Barcelona, Spain
Francisco J. Sanza
Center for Biomedical Technology, Optics, Photonics and Biophotonics Lab, Universidad Politécnica de Madrid. Campus Montegancedo, 28223 Pozuelo de Alarcón, Madrid, Spain
Rafael Casquel
Center for Biomedical Technology, Optics, Photonics and Biophotonics Lab, Universidad Politécnica de Madrid. Campus Montegancedo, 28223 Pozuelo de Alarcón, Madrid, Spain
The specificity and affinity of antibody-antigen interactions is a fundamental way to achieve reliable biosensing responses. Different proteins involved with dry eye dysfunction: ANXA1, ANXA11, CST4, PRDX5, PLAA and S100A6; were validated as biomarkers. In this work several antibodies were tested for ANXA1, ANXA11 and PRDX5 to select the best candidates for each biomarker. The results were obtained by using Biophotonic Sensing Cells (BICELLs) as an efficient methodology for label-free biosensing and compared with the Enzyme-Linked Immuno Sorbent Assay (ELISA) technique.
