Profiling of mouse macrophage lipidome using direct infusion shotgun mass spectrometry

Wei-Yuan Hsieh; Kevin J. Williams; Baolong Su; Steven J. Bensinger

STAR Protocols (Mar 2021)

Profiling of mouse macrophage lipidome using direct infusion shotgun mass spectrometry

Wei-Yuan Hsieh,
Kevin J. Williams,
Baolong Su,
Steven J. Bensinger

Affiliations

Wei-Yuan Hsieh: Department of Microbiology, Immunology and Molecular Genetics, University of California, Los Angeles, Los Angeles, CA 90095, USA; Corresponding author
Kevin J. Williams: Department of Biological Chemistry, University of California, Los Angeles, Los Angeles, CA 90095, USA; UCLA Lipidomics Lab, University of California, Los Angeles, Los Angeles, CA 90095, USA
Baolong Su: Department of Biological Chemistry, University of California, Los Angeles, Los Angeles, CA 90095, USA; UCLA Lipidomics Lab, University of California, Los Angeles, Los Angeles, CA 90095, USA
Steven J. Bensinger: Department of Microbiology, Immunology and Molecular Genetics, University of California, Los Angeles, Los Angeles, CA 90095, USA; UCLA Lipidomics Lab, University of California, Los Angeles, Los Angeles, CA 90095, USA; Corresponding author

Journal volume & issue: Vol. 2, no. 1
p. 100235

Abstract

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Summary: Immune cells, such as macrophages, reprogram their lipid metabolism in response to the activation of pattern recognition receptors (e.g., TLRs, NLRs) and cytokine receptors (e.g., interferons, interleukins). Profiling these changes can be achieved with shotgun mass spectrometry. This protocol provides step-by-step instructions on the generation and stimulation of bone marrow-derived macrophages (BMDMs), sample collection, and lipid extraction for profiling the macrophage lipidome.For complete details on the use and execution of this protocol, please refer to Hsieh et al. (2020).

Published in STAR Protocols

ISSN: 2666-1667 (Online)
Publisher: Elsevier
Country of publisher: United States
LCC subjects: Science: Science (General)
Website: https://star-protocols.cell.com/

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