Production of human recombinant neurotrophins for biomedical studies

Abstract

The genes of nerve growth factor (NGF), brain neurotrophic factor (BDNF) and human neurotrophic factor 3 (NT-3) were cloned and expressed in Escherichia coli. Methods of purification and renaturation of the proneurotrophins were developed. It was shown that the recombinant pro-NGF, pro-BDNF and pro-NT-3 induce a differentiation of chicken dorsal root ganglia cell culture.

Keywords

• expression
• renaturation
• recombinant human proneurotrophins
• prosequence
• chicken dorsal root ganglia