Green Synthesis of Silver Nanoparticles Using the Plant Extract of <i>Acer oblongifolium</i> and Study of Its Antibacterial and Antiproliferative Activity via Mathematical Approaches
Muhammad Naveed,
Bakhtawar Bukhari,
Tariq Aziz,
Sumera Zaib,
Muhammad Adil Mansoor,
Ayaz Ali Khan,
Muhammad Shahzad,
Anas S. Dablool,
Mashael W. Alruways,
Abdulraheem Ali Almalki,
Abdulhakeem S. Alamri,
Majid Alhomrani
Affiliations
Muhammad Naveed
Department of Biotechnology, Faculty of Life Sciences, University of Central Punjab, Lahore 54590, Pakistan
Bakhtawar Bukhari
Department of Biotechnology, Faculty of Life Sciences, University of Central Punjab, Lahore 54590, Pakistan
Tariq Aziz
Pak-Austria Fachhochschule, Institute of Applied Sciences and Technology, Mang, Haripur 22621, Pakistan
Sumera Zaib
Department of Biochemistry, Faculty of Life Sciences, University of Central Punjab, Lahore 54590, Pakistan
Muhammad Adil Mansoor
Department of Chemistry, School of Natural Sciences (SNS), National University of Sciences and Technology (NUST), H-12, Islamabad 44000, Pakistan
Ayaz Ali Khan
Department of Biotechnology, University of Malakand, Chakdara 18800, Pakistan
Muhammad Shahzad
Institute of Basic Medical Sciences, Khyber Medical University, Peshawar 25120, Pakistan
Anas S. Dablool
Department of Public Health, Health Sciences College Al-Leith, Umm Al-Qura University, Makkah al-Mukarramah 24382, Saudi Arabia
Mashael W. Alruways
Department of Clinical Laboratory Sciences, College of Applied Medical Sciences, Shaqra University, Shaqra 15273, Saudi Arabia
Abdulraheem Ali Almalki
Department of Clinical Laboratory Sciences, The Faculty of Applied Medical Sciences, Taif University, P.O. Box 11099, Taif 21944, Saudi Arabia
Abdulhakeem S. Alamri
Department of Clinical Laboratory Sciences, The Faculty of Applied Medical Sciences, Taif University, P.O. Box 11099, Taif 21944, Saudi Arabia
Majid Alhomrani
Department of Clinical Laboratory Sciences, The Faculty of Applied Medical Sciences, Taif University, P.O. Box 11099, Taif 21944, Saudi Arabia
In this study, the antibacterial and antifungal properties of silver nanoparticles synthesized with the aqueous plant extract of Acer oblongifolium leaves were defined using a simplistic, environmentally friendly, reliable, and cost-effective method. The aqueous plant extract of Acer oblongifolium, which served as a capping and reducing agent, was used to biosynthesize silver nanoparticles. UV visible spectroscopy, X-ray diffraction (XRD), Fourier Transform Infrared Spectroscopy (FTIR), and scanning electron microscopy were used to analyze the biosynthesized Acer oblongifolium silver nanoparticles (AgNPs). Gram-positive bacteria (Bacillus paramycoides and Bacillus cereus) and Gram-negative bacteria (E. coli) were used to test the AgNPs’ antibacterial activity. The presence of different functional groups was determined by FTIR. The AgNPs were rod-like in shape. The nanoparticles were more toxic against Escherichiacoli than both Bacillus cereus and Bacillus paramycoides. The AgNPs had IC50 values of 6.22 and 9.43 and mg/mL on HeLa and MCF-7, respectively, proving their comparatively strong potency against MCF-7. This confirmed that silver nanoparticles had strong antibacterial activity and antiproliferative ability against MCF-7 and HeLa cell lines. The mathematical modeling revealed that the pure nanoparticle had a high heat-absorbing capacity compared to the mixed nanoparticle. This research demonstrated that the biosynthesized Acer oblongifolium AgNPs could be used as an antioxidant, antibacterial, and anticancer agent in the future.
