Journal of Pain Research (Jan 2022)

Increased Brain-Derived Neurotrophic Factor Levels in Cerebrospinal Fluid During the Acute Phase in TBI-Induced Mechanical Allodynia in the Rat Model

  • Do W,
  • Baik J,
  • Jeon S,
  • You CM,
  • Kang D,
  • Jung YH,
  • Lee J,
  • Kim HK

Journal volume & issue
Vol. Volume 15
pp. 229 – 239

Abstract

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Wangseok Do,1 Jiseok Baik,1,2 Soeun Jeon,1 Chang-Min You,1 Dahyun Kang,1 Young-Hoon Jung,1 Jiyoon Lee,3 Hae-Kyu Kim1,2 1Department of Anesthesia and Pain Medicine and Biomedical Research Institute Pusan National University Hospital, Busan, Republic of Korea; 2Department of Anesthesia and Pain Medicine, School of Medicine, Pusan National University, Busan, Republic of Korea; 3Department of Anesthesia and Pain Medicine, Seoul National University Bundang Hospital, Seongnam-si, Gyeonggi-do, Republic of KoreaCorrespondence: Jiseok BaikDepartment of Anesthesia and Pain Medicine and Biomedical Research Institute Pusan National University Hospital, 179 Gudeok-Ro, Seo-gu, Busan, 49241, Republic of Korea, Tel +82-51-240-7499, Fax +82-51-242-7466, Email [email protected]: The present study aimed to develop a rat model for mechanical allodynia after traumatic brain injury (TBI) and to investigate the expression of brain-derived neurotrophic factor (BDNF) in the cerebrospinal fluid (CSF) using this model.Methods: A total of 180 rats were randomly allocated into three groups: a control group (group C), a sham-operated group (group S), and a controlled cortical impact induced TBI group (group T), 60 in each group. Von Frey test was performed to evaluate mechanical withdrawal thresholds. An enzyme-linked immunosorbent assay was performed to quantify BDNF level in CSF.Results: The 50% withdrawal thresholds of group T were lower than those of group C and group S at all measuring points except for the preoperative period (P = 0.026, < 0.001, and < 0.001 for POD1, POD7, and POD14, respectively). The BDNF level of group T was higher than those of group C and group S at POD1 (P = 0.005).Conclusion: Upregulation of the BDNF expression in CSF was observed in rats who developed mechanical allodynia on the day after TBI. Based on our findings, to elucidate the relationship between TBI-induced neuropathic pain and BDNF expression in CSF, further research should be carried out through a multifaceted approach to a broad spectrum of pain behavior models.Keywords: brain injuries, traumatic, brain injuries, brain-derived neurotrophic factor, nerve growth factors, neuralgia, models, animal

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