Evaluation of Pectimorf as growth regulator in the formation of callus with embriogenic structures in Ipomoea batatas

Abstract

Tuberous roots of sweet potato belonging to the clone INIVIT B 93-1, were gather ad and placed in flasks with water in the laboratory to induce the emergence of the yolks. Later on, explants of the limbo was selected to foliate (1 cm2) fields in the means of cultivation of Murashige and Skoog, 100 mg.l-1 myo-inositol, 1 mg.l-1 tiamine, 3% sucrose, 0.2% gelrite, 0.50 mg.l-1 2,4-D and 0.25 mg.l-1 6-BAP as control treatment and the same medium of above mentioned culture with different pectimorf concentrations (5.0, 10.0, 15.0 and 20.0 mg.l-1) for the formation of callus with embryogenic structures. Thirty days later the percentage of formed callus, their the callus growth, color, texture and presence of roots were evaluated. The callus stayed in incubation camera under conditions of darkness, temperature of 25 ± 2°C and relative humidity of 80-90%. Good results were obtained in the formation of callus with embryogenic structures, when used the pectimorf at 15.0 mg.l-1 concentration. Key works: embryogenesis, sweet potato, oligopectates