Nature Communications (Feb 2021)

Direct supercritical angle localization microscopy for nanometer 3D superresolution

  • Anindita Dasgupta,
  • Joran Deschamps,
  • Ulf Matti,
  • Uwe Hübner,
  • Jan Becker,
  • Sebastian Strauss,
  • Ralf Jungmann,
  • Rainer Heintzmann,
  • Jonas Ries

DOI
https://doi.org/10.1038/s41467-021-21333-x
Journal volume & issue
Vol. 12, no. 1
pp. 1 – 9

Abstract

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Supercritical angle localisation microscopy (SALM) allows the z-positions of single fluorophores to be extracted from the intensity of supercritical angle fluorescence. Here the authors improve the z-resolution of SALM, and report nanometre isotropic localisation precision on DNA origami structures.