Preparation of 20-HETE using multifunctional enzyme type 2-negative Starmerella bombicola[S]

Inge Van Bogaert; Guodong Zhang; Jun Yang; Jun-Yan Liu; Yonghao Ye; Wim Soetaert; Bruce D. Hammock

Journal of Lipid Research (Nov 2013)

Preparation of 20-HETE using multifunctional enzyme type 2-negative Starmerella bombicola[S]

Inge Van Bogaert,
Guodong Zhang,
Jun Yang,
Jun-Yan Liu,
Yonghao Ye,
Wim Soetaert,
Bruce D. Hammock

Affiliations

Inge Van Bogaert: Faculty of Bioscience Engineering, Ghent University, Ghent, Belgium
Guodong Zhang: To whom correspondence should be addressed. (B.D.H.); (G.Z.); Department of Entomology and Comprehensive Cancer Center, University of California-Davis, Davis, CA; Department of Food Science, University of Massachusetts-Amherst, Amherst, MA
Jun Yang: Department of Entomology and Comprehensive Cancer Center, University of California-Davis, Davis, CA
Jun-Yan Liu: Department of Entomology and Comprehensive Cancer Center, University of California-Davis, Davis, CA
Yonghao Ye: Jiangsu Key Laboratory of Pesticide Science, Nanjing Agricultural University, Nanjing, People's Republic of China
Wim Soetaert: Faculty of Bioscience Engineering, Ghent University, Ghent, Belgium
Bruce D. Hammock: To whom correspondence should be addressed. (B.D.H.); (G.Z.); Department of Entomology and Comprehensive Cancer Center, University of California-Davis, Davis, CA

Journal volume & issue: Vol. 54, no. 11
pp. 3215 – 3219

Abstract

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The metabolism of arachidonic acid (ARA) by cytochrome P450 ω/ω-1-hydroxylases leads to the formation of 20-hydroxyeicosatetraenoic acid (20-HETE), which is an important lipid-signaling molecule involved in regulation of vascular tone, angiogenesis, and inflammation. Development of a simple method to prepare 20-HETE would greatly facilitate the investigation of its biological activities. The nonpathogenic yeast Starmerella bombicola has been shown to convert exogenously added arachidonic acid to 20-HETE via the biosynthetic pathway of sophorolipids; however, the yield was low. Here we demonstrate that genetic knockout of multifunctional enzyme type 2 (MFE-2), which is involved in the β-oxidation of fatty acids, significantly increases the yield of ARA conversion to 20-HETE and allows practical preparation of 20-HETE.

Published in Journal of Lipid Research

ISSN: 0022-2275 (Print); 1539-7262 (Online)
Publisher: Elsevier
Country of publisher: United States
LCC subjects: Science: Chemistry: Organic chemistry: Biochemistry
Website: https://www.journals.elsevier.com/journal-of-lipid-research

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