STAR Protocols (Dec 2021)

Combining low-density cell culture, single-cell tracking, and patch-clamp to monitor the behavior of postnatal murine cerebellar neural stem cells

  • Aida Menéndez-Méndez,
  • Lucía Paniagua-Herranz,
  • Luis A. Olivos-Oré,
  • Rosa Gómez-Villafuertes,
  • Raquel Pérez-Sen,
  • Esmerilda G. Delicado,
  • Antonio R. Artalejo,
  • Felipe Ortega

Journal volume & issue
Vol. 2, no. 4
p. 100964

Abstract

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Summary: Low-density cell culture of the postnatal cerebellum, combined with live imaging and single-cell tracking, allows the behavior of postnatal cerebellar neural stem cells (NSCs) and their progeny to be monitored. Cultured cerebellar NSCs maintain their neurogenic nature giving rise, in the same relative proportions that exist in vivo, to the neuronal progeny generated by the three postnatal cerebellar neurogenic niches. This protocol describes the identification of the nature of the progeny through both post-imaging immunocytochemistry and patch-clamp recordings.For complete details on the use and execution of this protocol, please refer to Paniagua-Herranz et al. (2020b).

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