Shanxian granule ameliorates diethylnitrosamine-induced precancerous lesions in liver by regulating inflammatory mediators

Abstract

To explore the effects of Shanxian Granules (SXG) on diethylnitrosamine (DEN) -induced precancerous lesions in rats and its possible molecular mechanism. A total of 50 male SD rats (190±10 g) were randomly divided into five groups. control group, DEN group, SXG low dose group, SXG medium dose group and SXG high dose group. The control group received saline injection. The precancerous liver cirrhosis group received 50mg/kg DEN via intraperitoneal injection twice a week for 16 weeks. The indexes of liver function (ALT, AST and GGT) were measured by blood sampling. HE staining was used to observe pathological changes of liver tissues. The levels of IL-6 and TNFα were measured by ELISA kits. Compared with the model group, the high (P<0.01) and middle dose (P<0.05) of SXG significantly reduced the damage of liver function and ameliorated the degree of liver disease, such as hepatic fibrosis. In addition, SXG significantly decreased the levels of IL-10 and TNFα in the liver of DEN-induced precancerous lesions. In conclusion, SXG can effectively and dose-dependently alleviate the morphological changes of rat liver precancerous lesions. and the underlying protective mechanism of SXG may be by inhibiting the release of inflammatory mediators, such as IL-6 and TNF-α.