Nature Communications (Jun 2016)

Efficient generation of monoclonal antibodies against peptide in the context of MHCII using magnetic enrichment

  • Justin A. Spanier,
  • Daniel R. Frederick,
  • Justin J. Taylor,
  • James R. Heffernan,
  • Dmitri I. Kotov,
  • Tijana Martinov,
  • Kevin C. Osum,
  • Jenna L. Ruggiero,
  • Blake J. Rust,
  • Samuel J. Landry,
  • Marc K. Jenkins,
  • James B. McLachlan,
  • Brian T. Fife

DOI
https://doi.org/10.1038/ncomms11804
Journal volume & issue
Vol. 7, no. 1
pp. 1 – 11

Abstract

Read online

Generating antibodies specific for the peptide–MHCII complexes has been challenging, with only a handful made to date. Here, the authors develop a more efficient approach to generate these antibodies, and demonstrate their potential in research and therapeutic applications.