An in vitro culture platform for studying the effect of collective cell migration on spatial self-organization within induced pluripotent stem cell colonies

Abstract

Highlights • Ring culture system induces distinct changes in actomyosin organization at the iPSC colony edge. • Removal of ring culture system induces collective cell migration at the iPSC colony edge. • Collective cell migration modulates self-organized fate patterning decisions in iPSC-derived gastrulation-stage meso–endoderm. • Cadherin switching during collective cell migration enhances endodermal differentiation.

Keywords

• Human induced pluripotent stem cells
• Ring culture system
• Actin actomyosin
• Collective cell migration
• Epithelial mesenchymal transition
• Mesendoderm lineage segregation