ISOLATION AND IDENTIFICATION OF PHOSPHATE SOLUBILIZING BACTERIA AND HYDROCARBON DEGRADATION BACTERIA IN LAPINDO MUD SIDOARJO - EAST JAVA - INDONESIA

Abstract

Lapindo mud, the biggest mud disaster in Sidoarjo, Indonesia since 2006, still offers numbers of constituents such as heavy metals and crude oil. The oil is produced by a mixture of complex hydrocarbon with organic compounds from sulfure, oxygen, nitrogen and metal. Moreover, those organic compounds can be used as substrates for bacteria growth. Thus, this paper aims at isolating and identifying potential bacteria in dissolving phosphate and degrading hydrocarbon in Lapindo mud. The characterization of phosphate solubilizing bacteria is obtained by using Pikovskaya media, in which a clear zone indicating potential phosphate solubilizing bacteria. The bacteria identification includes macroscopic observation in a bacteria colony, gram-coloring bacteria and physiological tests. Under descriptive analysis, results show that there are three species namely Pseudomonas pseudomallei, Pseudomonas fluorescens35, and Pseudomonas stutzeri with the percentages of probability 99.99%; 58.26% and 66.40% respectively.

Keywords

• Hydrocarbon degradation bacteria
• Isolation and identification
• Lapindo
• Phosphate solubilizing bacteria