ROLUL UNOR AGENŢI CHIMICI ALCHILANŢI ÎN MORFOGENEZA IN VITRO A UNOR SPECII. IMPLICAŢIILE LOR ÎN AMELIORARE ŞI ECOLOGIE

Abstract

The paper deals with the in vitro behaviour of some explants (apex, node), prevailed from the esparcet species Onobrichis vicifolia Scop., for the regeneration of species, their multiplication and application of a mutagen (table 1). The apex regenerated plants completely formed with multiplication V2 and V3 (media that proved to be the best). The node also generated neo plantlets completely confirmed with a high regenerative capacity on almost all variants. On media with Zeatin the regeneration percentage reached 95 – 99%. The media with 2,4 D produced a callus from both explants, the biggest mass of embryogen callus, with visible embryos and a regenerative capacity were obtained from apex V7 and V9. The embryogen callus treated with mutagen medium in low concentration showed a good regenerative capacity especially on media with dimethylsulphonate (DMS)