Deciphering the mechanism of processive ssDNA digestion by the Dna2-RPA ensemble

Jiangchuan Shen; Yiling Zhao; Nhung Tuyet Pham; Yuxi Li; Yixiang Zhang; Jonathan Trinidad; Grzegorz Ira; Zhi Qi; Hengyao Niu

doi:10.1038/s41467-021-27940-y

Nature Communications (Jan 2022)

Deciphering the mechanism of processive ssDNA digestion by the Dna2-RPA ensemble

Jiangchuan Shen,
Yiling Zhao,
Nhung Tuyet Pham,
Yuxi Li,
Yixiang Zhang,
Jonathan Trinidad,
Grzegorz Ira,
Zhi Qi,
Hengyao Niu

Affiliations

Jiangchuan Shen: Department of Molecular and Cellular Biochemistry, Indiana University
Yiling Zhao: Center for Quantitative Biology, Peking-Tsinghua Center for Life Sciences, Academy for Advanced Interdisciplinary Studies, Peking University
Nhung Tuyet Pham: Department of Molecular and Human Genetics, Baylor College of Medicine
Yuxi Li: Department of Molecular and Cellular Biochemistry, Indiana University
Yixiang Zhang: Department of Chemistry, Biological Mass Spectrometry Facility, Indiana University
Jonathan Trinidad: Department of Chemistry, Biological Mass Spectrometry Facility, Indiana University
Grzegorz Ira: Department of Molecular and Human Genetics, Baylor College of Medicine
Zhi Qi: Center for Quantitative Biology, Peking-Tsinghua Center for Life Sciences, Academy for Advanced Interdisciplinary Studies, Peking University
Hengyao Niu: Department of Molecular and Cellular Biochemistry, Indiana University

DOI: https://doi.org/10.1038/s41467-021-27940-y
Journal volume & issue: Vol. 13, no. 1
pp. 1 – 16

Abstract

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RPA protects the integrity of single stranded DNA during DNA repair processes. Here the authors show how RPA actively participates in DNA transactions through its interactions with the endonuclease Dna2.

Published in Nature Communications

ISSN: 2041-1723 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science
Website: https://www.nature.com/ncomms/

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