Isolation and characterization of a novel human intestinal Enterococcus faecium FUA027 capable of producing urolithin A from ellagic acid

Xiaomeng Zhang; Xiaomeng Zhang; Xiaomeng Zhang; Yaowei Fang; Yaowei Fang; Yaowei Fang; Guang Yang; Guang Yang; Guang Yang; Xiaoyue Hou; Xiaoyue Hou; Xiaoyue Hou; Yang Hai; Mengjie Xia; Fuxiang He; Yaling Zhao; Yaling Zhao; Yaling Zhao; Shu Liu; Shu Liu; Shu Liu

doi:10.3389/fnut.2022.1039697

Frontiers in Nutrition (Nov 2022)

Isolation and characterization of a novel human intestinal Enterococcus faecium FUA027 capable of producing urolithin A from ellagic acid

Xiaomeng Zhang,
Xiaomeng Zhang,
Xiaomeng Zhang,
Yaowei Fang,
Yaowei Fang,
Yaowei Fang,
Guang Yang,
Guang Yang,
Guang Yang,
Xiaoyue Hou,
Xiaoyue Hou,
Xiaoyue Hou,
Yang Hai,
Mengjie Xia,
Fuxiang He,
Yaling Zhao,
Yaling Zhao,
Yaling Zhao,
Shu Liu,
Shu Liu,
Shu Liu

Affiliations

Xiaomeng Zhang: Co-Innovation Center of Jiangsu Marine Bio-industry Technology, Jiangsu Ocean University, Lianyungang, China
Xiaomeng Zhang: Jiangsu Key Laboratory of Marine Bioresources and Environment/Jiangsu Key Laboratory of Marine Biotechnology, Jiangsu Ocean University, Lianyungang, China
Xiaomeng Zhang: School of Food Science and Engineering, Jiangsu Ocean University, Lianyungang, China
Yaowei Fang: Co-Innovation Center of Jiangsu Marine Bio-industry Technology, Jiangsu Ocean University, Lianyungang, China
Yaowei Fang: Jiangsu Key Laboratory of Marine Bioresources and Environment/Jiangsu Key Laboratory of Marine Biotechnology, Jiangsu Ocean University, Lianyungang, China
Yaowei Fang: School of Food Science and Engineering, Jiangsu Ocean University, Lianyungang, China
Guang Yang: Co-Innovation Center of Jiangsu Marine Bio-industry Technology, Jiangsu Ocean University, Lianyungang, China
Guang Yang: Jiangsu Key Laboratory of Marine Bioresources and Environment/Jiangsu Key Laboratory of Marine Biotechnology, Jiangsu Ocean University, Lianyungang, China
Guang Yang: School of Food Science and Engineering, Jiangsu Ocean University, Lianyungang, China
Xiaoyue Hou: Co-Innovation Center of Jiangsu Marine Bio-industry Technology, Jiangsu Ocean University, Lianyungang, China
Xiaoyue Hou: Jiangsu Key Laboratory of Marine Bioresources and Environment/Jiangsu Key Laboratory of Marine Biotechnology, Jiangsu Ocean University, Lianyungang, China
Xiaoyue Hou: School of Food Science and Engineering, Jiangsu Ocean University, Lianyungang, China
Yang Hai: Key Laboratory of Marine Drugs, School of Medicine and Pharmacy, The Ministry of Education of China, Ocean University of China, Qingdao, China
Mengjie Xia: School of Food Science and Engineering, Jiangsu Ocean University, Lianyungang, China
Fuxiang He: School of Food Science and Engineering, Jiangsu Ocean University, Lianyungang, China
Yaling Zhao: Co-Innovation Center of Jiangsu Marine Bio-industry Technology, Jiangsu Ocean University, Lianyungang, China
Yaling Zhao: Jiangsu Key Laboratory of Marine Bioresources and Environment/Jiangsu Key Laboratory of Marine Biotechnology, Jiangsu Ocean University, Lianyungang, China
Yaling Zhao: School of Food Science and Engineering, Jiangsu Ocean University, Lianyungang, China
Shu Liu: Co-Innovation Center of Jiangsu Marine Bio-industry Technology, Jiangsu Ocean University, Lianyungang, China
Shu Liu: Jiangsu Key Laboratory of Marine Bioresources and Environment/Jiangsu Key Laboratory of Marine Biotechnology, Jiangsu Ocean University, Lianyungang, China
Shu Liu: School of Food Science and Engineering, Jiangsu Ocean University, Lianyungang, China

DOI: https://doi.org/10.3389/fnut.2022.1039697
Journal volume & issue: Vol. 9

Abstract

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Urolithin A (UA) has received considerable research attention because of its health benefits. However, only a few strains have been reported to produce UA from ellagic acid (EA), and the molecular mechanisms underlying the gut microbiota-mediated transformation of ellagic acid into urolithin A is limited. In the present study, a single strain FUA027 capable of converting ellagic acid into UA in vitro was isolated from the fecal samples. The strain was identified as Enterococcus faecium through the morphological, physiological, biochemical and genetic tests. UA was produced at the beginning of the stationary phase and its levels peaked at 50 h, with the highest concentration being 10.80 μM. The strain Enterococcus faecium FUA027 is the first isolated strain of Enterococcus sp. producing urolithin A from ellagic acid, which may be developed as probiotics and used to explore molecular mechanisms underlying the biotransformation of ellagic acid into UA.

Published in Frontiers in Nutrition

ISSN: 2296-861X (Online)
Publisher: Frontiers Media S.A.
Country of publisher: Switzerland
LCC subjects: Technology: Home economics: Nutrition. Foods and food supply
Website: https://www.frontiersin.org/journals/nutrition/

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