Investigation of the Effects of antiepileptics On Mitotic Proliferation in Glioblastoma and Neuroblastoma In Vitro Cell Cultures

Abstract

Objective:The frequency of epileptic seizures is high in patients with brain tumors and its treatment is important. It is atypical mitotic proliferation that is effective in the proliferation of malignant tumor cells. It is known that antiepileptics have direct or indirect effects on mitotic proliferation. In our study, we aimed to maximize the use of both the antiepileptic effect and the cytoreductive effect by suppressing tumor growth while choosing the drug to stop the seizure.Methods:In our study, anti-tumoral activities of antiepileptic agents containing gabapentin, pregabalin, valproic acid, levetiracetam, zonisamide, phenytoin, carbamazepine in in vitro glioblastoma (c6) and neuroblastoma cell cultures (NA/An1) were evaluated with a real-time cell analysis system. Statistically, the difference between groups was investigated by analysis of variance, followed by post hoc Tukey’s test Statistical Package for the Social Sciences software 16.0 (IBM Inc, Chicago, IL, USA).Results:In our study, it was observed that all drugs except gabapentin had antimitotic effects in glioblastoma cell cultures, among which phenytoin, levetiracetam, and valproic acid had dose-dependent antimitotic effects, while carbamazepine had reduced antimitotic effects at concentrations above 25 μg/mL. In in vitro neuroblastoma cell cultures, it was found that only valproic acid and zonisamide had antimitotic effects, and other drugs studied did not have antimitotic effects.Conclusions:In our study, it was observed that the preference of antiepileptics with a high antimitotic effect on tumoral cells was important when arranging seizure treatment in patients with brain tumors.

Keywords

• antiepileptics
• cell culture
• cytotoxicity
• glioblastoma
• mitotic proliferation
• neuroblastoma
• xcelligence