Bone Reports (Jun 2025)

Bone mechanical loading reduces heart rate and increases heart rate variability in mice

  • Julian A. Vallejo,
  • Mark Gray,
  • Jackson Klump,
  • Andrew Wacker,
  • Mark Dallas,
  • Mark L. Johnson,
  • Michael J. Wacker

DOI
https://doi.org/10.1016/j.bonr.2025.101844
Journal volume & issue
Vol. 25
p. 101844

Abstract

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Cardiovascular disease and osteoporosis are clinically associated. Bone adapts to mechanical forces by altering its overall structure and mass. In response to mechanical strain bone cells release signaling molecules and activate the nervous system. Bone also exhibits endocrine functions that modulate a number of tissues including the heart. We hypothesized that bone mechanical loading acutely alters cardiac function via neural and/or endocrine mechanisms. To test this hypothesis, we performed in vivo tibia mechanical loading in anesthetized mice while monitoring heart parameters using electrocardiogram (ECG). An immediate, transient reduction in resting heart rate was observed during tibial loading in both adult male and female mice (p 0.05). This suggests that a neuronal afferent pathway in the hindlimb and reduction in efferent sympathetic tone mediate this bone-neuro-heart reflex. In conclusion, the findings that tibia bone loading age-dependently modulates heart function support the concept of physiological coupling of the skeletal and cardiovascular systems.

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