Bone mechanical loading reduces heart rate and increases heart rate variability in mice
Julian A. Vallejo,
Mark Gray,
Jackson Klump,
Andrew Wacker,
Mark Dallas,
Mark L. Johnson,
Michael J. Wacker
Affiliations
Julian A. Vallejo
University of Missouri – Kansas City, School of Medicine, Department of Biomedical Sciences, USA; University of Missouri – Kansas City, School of Dentistry, Department of Oral & Craniofacial Sciences, USA
Mark Gray
University of Missouri – Kansas City, School of Medicine, Department of Biomedical Sciences, USA
Jackson Klump
University of Missouri – Kansas City, School of Medicine, Department of Biomedical Sciences, USA
Andrew Wacker
University of Missouri – Kansas City, School of Medicine, Department of Biomedical Sciences, USA
Mark Dallas
University of Missouri – Kansas City, School of Dentistry, Department of Oral & Craniofacial Sciences, USA
Mark L. Johnson
University of Missouri – Kansas City, School of Dentistry, Department of Oral & Craniofacial Sciences, USA
Michael J. Wacker
University of Missouri – Kansas City, School of Medicine, Department of Biomedical Sciences, USA; Corresponding author at: 2411 Holmes, Kansas City, MO 64108, USA.
Cardiovascular disease and osteoporosis are clinically associated. Bone adapts to mechanical forces by altering its overall structure and mass. In response to mechanical strain bone cells release signaling molecules and activate the nervous system. Bone also exhibits endocrine functions that modulate a number of tissues including the heart. We hypothesized that bone mechanical loading acutely alters cardiac function via neural and/or endocrine mechanisms. To test this hypothesis, we performed in vivo tibia mechanical loading in anesthetized mice while monitoring heart parameters using electrocardiogram (ECG). An immediate, transient reduction in resting heart rate was observed during tibial loading in both adult male and female mice (p 0.05). This suggests that a neuronal afferent pathway in the hindlimb and reduction in efferent sympathetic tone mediate this bone-neuro-heart reflex. In conclusion, the findings that tibia bone loading age-dependently modulates heart function support the concept of physiological coupling of the skeletal and cardiovascular systems.