Age quadratically affects intestinal calcium and phosphorus transporter gene expression in broiler chickens

Xianliang Lv; Junfang Hao; Lihua Wu; Mengyuan Liu; Lei He; Yingying Qiao; Yanyan Cui; Guan Wang; Chunmei Zhang; Hongxia Qu; Jincheng Han

doi:10.5713/ab.22.0058

Animal Bioscience (Dec 2022)

Age quadratically affects intestinal calcium and phosphorus transporter gene expression in broiler chickens

Xianliang Lv,
Junfang Hao,
Lihua Wu,
Mengyuan Liu,
Lei He,
Yingying Qiao,
Yanyan Cui,
Guan Wang,
Chunmei Zhang,
Hongxia Qu,
Jincheng Han

Affiliations

Xianliang Lv: College of Animal Science and Technology, Henan Agricultural University, Zhengzhou 450046, China
Junfang Hao: Department of Animal Science, College of Biology and Food, Shangqiu Normal University, Shangqiu 476000, China
Lihua Wu: College of Animal Science and Technology, Henan Agricultural University, Zhengzhou 450046, China
Mengyuan Liu: College of Animal Science and Technology, Henan Agricultural University, Zhengzhou 450046, China
Lei He: Department of Animal Science, College of Biology and Food, Shangqiu Normal University, Shangqiu 476000, China
Yingying Qiao: Department of Biochemistry and Biotechnology, Sumy National Agrarian University, Sumy 40000, Ukraine
Yanyan Cui: Department of Animal Science, College of Biology and Food, Shangqiu Normal University, Shangqiu 476000, China
Guan Wang: Department of Animal Science, College of Biology and Food, Shangqiu Normal University, Shangqiu 476000, China
Chunmei Zhang: Department of Animal Science, College of Biology and Food, Shangqiu Normal University, Shangqiu 476000, China
Hongxia Qu: Department of Animal Science, College of Biology and Food, Shangqiu Normal University, Shangqiu 476000, China
Jincheng Han: Department of Animal Science, College of Biology and Food, Shangqiu Normal University, Shangqiu 476000, China

DOI: https://doi.org/10.5713/ab.22.0058
Journal volume & issue: Vol. 35, no. 12
pp. 1921 – 1928

Abstract

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Objective This research aimed to evaluate the effects of age on growth, tibia development, and intestinal calcium (Ca) and phosphorus (P) transporter gene expressions in broiler chickens. Methods A total of 224 male Arbor Acres broilers were fed with nutrient-adequate diets and reared in eight cages (28 broilers per cage). Eight broilers (one broiler per cage) were selected and killed at 5, 10, 15, 20, 25, 30, 35, and 40 days of age, respectively. Results Body weight continuously increased with age of broiler chickens from 5 to 40 days. The bone weight, ash weight, diameter, and length of the tibia also increased with broiler age. By contrast, the tibia ash, Ca, and P percentages quadratically changed with age (p<0.001), and the highest values of mineral contents were observed at 20, 25, and 25 days of age, respectively. The mRNA abundances of calcium-binding protein 28-kDa (CaBP-D28k), sodium-calcium exchanger 1 (NCX1), and plasma membrane ATPase 1b (PMCA1b) increased from 5 to 25 days and then decreased up to 40 days. Similar results were noted in the mRNA abundances of IIb sodium-phosphate cotransporter (NaPi-IIb), inorganic phosphate transporter 1 (PiT-1), inorganic phosphate transporter 2 (PiT-2), nuclear vitamin D receptor (nVDR), and membrane vitamin D receptor (mVDR). The mRNA abundances of Ca and P transporters and VDRs were the highest at 25 days of age. Conclusion These data indicate that age quadratically affects intestinal Ca and P transporter gene expression and mineral absorption capacity in broiler chickens.

Published in Animal Bioscience

ISSN: 2765-0189 (Print); 2765-0235 (Online)
Publisher: Asian-Australasian Association of Animal Production Societies
Country of publisher: Korea, Republic of
LCC subjects: Science: Zoology
Website: https://www.animbiosci.org/index.php

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