Precise Deposition of Polydopamine on Cancer Cell Membrane as Artificial Receptor for Targeted Drug Delivery
Hoda Safari Yazd,
Yu Yang,
Long Li,
Lu Yang,
Xiaowei Li,
Xiaoshu Pan,
Zhuo Chen,
Jianhui Jiang,
Cheng Cui,
Weihong Tan
Affiliations
Hoda Safari Yazd
Department of Chemistry, University of Florida, Gainesville, FL 32611-7200, USA
Yu Yang
Molecular Science and Biomedicine Laboratory (MBL), State Key Laboratory for Chemo/BioSensing and Chemometrics, College of Chemistry and Chemical Engineering, College of Life Sciences, and Aptamer Engineering Center of Hunan Province, Hunan University, Changsha 410082, China; Institute of Molecular Medicine (IMM), Renji Hospital, Shanghai Jiao Tong University School of Medicine, and College of Chemistry and Chemical Engineering, Shanghai Jiao Tong University, Shanghai 200240, China
Long Li
Department of Chemistry, University of Florida, Gainesville, FL 32611-7200, USA
Lu Yang
Department of Chemistry, University of Florida, Gainesville, FL 32611-7200, USA
Xiaowei Li
Department of Chemistry, University of Florida, Gainesville, FL 32611-7200, USA
Xiaoshu Pan
Department of Chemistry, University of Florida, Gainesville, FL 32611-7200, USA
Zhuo Chen
Molecular Science and Biomedicine Laboratory (MBL), State Key Laboratory for Chemo/BioSensing and Chemometrics, College of Chemistry and Chemical Engineering, College of Life Sciences, and Aptamer Engineering Center of Hunan Province, Hunan University, Changsha 410082, China
Jianhui Jiang
Molecular Science and Biomedicine Laboratory (MBL), State Key Laboratory for Chemo/BioSensing and Chemometrics, College of Chemistry and Chemical Engineering, College of Life Sciences, and Aptamer Engineering Center of Hunan Province, Hunan University, Changsha 410082, China
Cheng Cui
Molecular Science and Biomedicine Laboratory (MBL), State Key Laboratory for Chemo/BioSensing and Chemometrics, College of Chemistry and Chemical Engineering, College of Life Sciences, and Aptamer Engineering Center of Hunan Province, Hunan University, Changsha 410082, China
Weihong Tan
Molecular Science and Biomedicine Laboratory (MBL), State Key Laboratory for Chemo/BioSensing and Chemometrics, College of Chemistry and Chemical Engineering, College of Life Sciences, and Aptamer Engineering Center of Hunan Province, Hunan University, Changsha 410082, China; Institute of Molecular Medicine (IMM), Renji Hospital, Shanghai Jiao Tong University School of Medicine, and College of Chemistry and Chemical Engineering, Shanghai Jiao Tong University, Shanghai 200240, China; Corresponding author
Summary: Compared with conventional chemotherapy and radiotherapy, targeted molecular therapy, e.g., antibody-drug conjugates or aptamer-drug conjugates, can specifically identify overexpressed natural receptors on the cancer cell, perform targeted release of anticancer drugs, and achieve targeted killing of tumor cells. However, many natural receptors are also expressed on non-cancer cells, thereby diverting the targeting molecules to healthy cells. By generating artificial cell surface receptors specific to diseased cells, aptamer-drug conjugates can identify these artificial receptors, improve therapeutic efficacy, and decrease the minimum effective dosage. In this study, we use high K+ and high H2O2 of the tumor microenvironment (TME) to produce polydopamine only on living cancer cell membrane. Owing to the significant reactivity of polydopamine with amino groups, e.g., the amino group of proteins, polydopamine can deposit on tumor cells and act as “artificial receptors” for targeted delivery of anticancer drugs with amino groups, in other words, amino-containing drugs and protein drugs.
