Uso de equações lineares na determinação dos parâmetros de Michaelis-Menten Use of linear equations to obtain Michaelis-Menten parameters

Abstract

The Michaelis-Menten equation is used in many biochemical and bioinorganic kinetic studies involving homogeneous catalysis. Otherwise, it is known that determination of Michaelis-Menten parameters K M, Vmax, and k cat by the well-known Lineweaver-Burk double reciprocal linear equation does not produce the best values for these parameters. In this paper we present a discussion on different linear equations which can be used to calculate these parameters and we compare their results with the values obtained by the more reliable nonlinear least-square fit.

Keywords

• Michaelis-Menten
• Lineweaver-Burk
• nonlinear least-square fit