International Journal Bioautomation (Jun 2018)
Application of a Microfluidic Chip with Mechanical Stimuli for Culturing Mouse Embryos in vitro
Abstract
In order to solve some problems of lower blastocyst rate of early embryos in vitro and poor quality of blastocyst, application of a microfluidic chip with mechanical stimuli (MS) to imitate microenvironment of fallopian tube, to investigate the effect of a microfluidic chip with mechanical stimuli on the early embryo development in mammalian. We designed and made a novel and simple system to apply MS similar to those generated inside the oviduct to cultured mammalian embryos by using poly(dimethylsiloxiane). Using this microfluidic chip embryo culture device to culture 2-cell stage mouse embryo from in vitro fertilization. There were large differences in development rates of > 4-cell stage, development rates of morula (63.1 ± 12.5% vs. 80.6 ± 17.9%) and blastocyst (58.6 ± 2.3% vs. 73.2 ± 2.8%) between control and microfluidic chip group (p > 0.05). meanwhile, there was large differences in cell numbers of inner cell mass in blastocyst between control and microfluidic chip group (p > 0.05). These results suggested that the culture device can significantly improve blastocyst development rate of embryo in vitro and the quality of the blastocyst in mice, this novel and simple system was feasible to generate and apply MS similar to those generated inside the oviduct to cultured mammalian embryos
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