Acta Medica Medianae (Apr 2006)
EFFECT OF PUTRESCINE ON INTENSITY OF LIPID PEROXIDATION IN RATS’ BRAIN WITH CHOLESTASIS
Abstract
Encephalopathy in cholestasis results from accumulation of unconjugated bilirubin (UCB) and hydrophobic bile acids (BA). Toxic BA and UCB induces neurotoxicity (apoptosis of neurons). Putrescine, spermidine and spermine are endogenous polyamines essential for cellular growth, regeneration and differen-tiation. Beneficial effects of putrescine in CNS injury have been attributed to anti-apoptotic ant anti-oxidant properties.The aim of the study was to examine the effect of putrescine at the level of lipid peroxidation in cholestatic brain injury.Wistar rats were divided into 5 groups: I-control, II-sham operated rats, III-treated only with putrescine, IV-bile duct ligated (BDL) rats, V-BDL rats treated with putrescine (150mg/kg BW ip.). The animals were sacrificed after the 9 -day treatment.Administration of putrescine in BDL rats reduces concentration of blood plasma UCB and BA (29.2±3.3 vs. 43.6±5.9 μmol/l and 11.4±0.8 vs. 22.8±2.6 μmol/l; p < 0.001). The lipid peroxidation (MDA) was increased in brain of BDL rats (4.98±0.54 vs. sham operated rats 3.99±0.32 nmol/mg prot; p < 0.001). Putrescine decreased MDA level in brain of V group vs. IV group rats (2.25±0.42 vs. 4.98±0.54 nmol/mg p; p < 0.001). The amplification of cerebral activity of polyamine oxidase (PAO) in BDL rats (1.25±0.09 vs. sham operated rats 0.81±0.09 U/mg prot; p < 0.001), resulted in high local concentrations of 3-acetamidopropanol and H2O2 which lead to oxidative stress and cell death. Administration of putrescine of BDL rats, decreased activity of cerebral PAO compared with BDL rats (1.02±0.07 vs. 1.25±0.09 U/mg prot; p < 0.001).Administration of putrescine in BDL rats results in normalization of cerebral oxidative stress and has protective role after the CNS injury in cholestasis.
