Larvicidal effect of zirconium oxide nanoparticles in protoscoleces of hydatid cysts in the laboratory and mice

Abstract

The research focuses on the effect of zirconium oxide nanoparticles in the activity of protoscoleces of Echinococcus granulosus in the lab., seven strengths of the solution have been employed, 2.5, 5, 10, 15, 25, 50, and 100 µg/milliliter in distinct exposition durations, 10, 20, 30, 60, 90 minutes. Scottish mice have been inoculated with PSCs exposed to zirconium oxide nanoparticles of 5, 10, 50, and 100 ug/milliliter, for 30 minutes. Unimposing protoscoleces to ZrO2 nanoparticles were administered to another group of animals as a controlling series. The whole experimental series has been anatomized after 3, 4, and 5 months after infestation. The results illustrated the apparent impact of zirconium oxide on the liveliness of protoscoleces, by increasing exposure time and strength concentricity in the laboratory, predominantly 29 and 0% at a concentration of 100 ug/ml at 60, and 90 minutes, distinctly. Likewise considerable diminished numbering of rising cysts in treated mice, mainly, at a concentration of 50 and 100 ug/ml in the fourth and fifth months 89.53 and 90.21% of invasion, with a presumed decrease in the diameters and weights of cysts in treated mice, exceptionally in the fifth month, in comparison with the untreated group. These observations presumed ZrO2 nanoparticles, as a promising alternative agent against hydatid disease through future supplementary research.

Keywords

• hydatidosis
• nanomaterials
• metacestodes