Genome-Wide Differential Expression Profiling of Ovarian circRNAs Associated With Litter Size in Pigs

Gaoxiao Xu; Gaoxiao Xu; Huifang Zhang; Xiao Li; Jianhong Hu; Gongshe Yang; Shiduo Sun

doi:10.3389/fgene.2019.01010

Frontiers in Genetics (Nov 2019)

Genome-Wide Differential Expression Profiling of Ovarian circRNAs Associated With Litter Size in Pigs

Gaoxiao Xu,
Gaoxiao Xu,
Huifang Zhang,
Xiao Li,
Jianhong Hu,
Gongshe Yang,
Shiduo Sun

Affiliations

Gaoxiao Xu: Key Laboratory of Animal Genetics, Breeding and Reproduction of Shaanxi Province, College of Animal Sciences and Technologies, Northwest A&F University, Yangling, China
Gaoxiao Xu: Teaching and Research Section of Biotechnology, Nanning University, Nanning, China
Huifang Zhang: Key Laboratory of Animal Genetics, Breeding and Reproduction of Shaanxi Province, College of Animal Sciences and Technologies, Northwest A&F University, Yangling, China
Xiao Li: Key Laboratory of Animal Genetics, Breeding and Reproduction of Shaanxi Province, College of Animal Sciences and Technologies, Northwest A&F University, Yangling, China
Jianhong Hu: Key Laboratory of Animal Genetics, Breeding and Reproduction of Shaanxi Province, College of Animal Sciences and Technologies, Northwest A&F University, Yangling, China
Gongshe Yang: Key Laboratory of Animal Genetics, Breeding and Reproduction of Shaanxi Province, College of Animal Sciences and Technologies, Northwest A&F University, Yangling, China
Shiduo Sun: Key Laboratory of Animal Genetics, Breeding and Reproduction of Shaanxi Province, College of Animal Sciences and Technologies, Northwest A&F University, Yangling, China

DOI: https://doi.org/10.3389/fgene.2019.01010
Journal volume & issue: Vol. 10

Abstract

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Circular RNAs (circRNAs) have been emerging as an important regulator in mammalian reproduction via acting as miRNA sponges. However, the circRNAs in porcine ovaries related with litter size remains largely unknown. In this study, porcine ovaries with smaller or larger litter size (LLS) were subjected to high-throughput RNA sequencing. In total, 38,722 circRNAs were identified, of which 1,291 circRNAs were commonly expressed in all samples. There were 56 circRNAs significantly down-regulated and 54 circRNAs up-regulated in LLS pig (|log2 (fold change) | > 1, FDR < 0.05). Bioinformatics predicted that most of circRNAs harbored miRNA binding sites, and the expression patterns of circRNAs and their putative binding miRNAs were validated by qPCR. Moreover, the expression of circ-TCP11/miR-183 was significantly reversely correlated and their direct interaction was confirmed by dual-luciferase assay. Our study indicates that circRNAs may play potential effects on modulating porcine litter size.

Published in Frontiers in Genetics

ISSN: 1664-8021 (Online)
Publisher: Frontiers Media S.A.
Country of publisher: Switzerland
LCC subjects: Science: Biology (General): Genetics
Website: http://journal.frontiersin.org/journal/genetics

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