Single-molecule long-read sequencing reveals a conserved intact long RNA profile in sperm

Yu H. Sun; Anqi Wang; Chi Song; Goutham Shankar; Rajesh K. Srivastava; Kin Fai Au; Xin Zhiguo Li

doi:10.1038/s41467-021-21524-6

Nature Communications (Mar 2021)

Single-molecule long-read sequencing reveals a conserved intact long RNA profile in sperm

Yu H. Sun,
Anqi Wang,
Chi Song,
Goutham Shankar,
Rajesh K. Srivastava,
Kin Fai Au,
Xin Zhiguo Li

Affiliations

Yu H. Sun: Center for RNA Biology: From Genome to Therapeutics, University of Rochester Medical Center
Anqi Wang: Department of Internal Medicine, University of Iowa
Chi Song: College of Public Health, Division of Biostatistics, The Ohio State University
Goutham Shankar: Center for RNA Biology: From Genome to Therapeutics, University of Rochester Medical Center
Rajesh K. Srivastava: Department of Obstetrics/Gynecology, University of Rochester Medical Center
Kin Fai Au: Department of Internal Medicine, University of Iowa
Xin Zhiguo Li: Center for RNA Biology: From Genome to Therapeutics, University of Rochester Medical Center

DOI: https://doi.org/10.1038/s41467-021-21524-6
Journal volume & issue: Vol. 12, no. 1
pp. 1 – 12

Abstract

Read online

Sperm carry diverse RNAs to the zygote. Here the authors employ single molecule long-read sequencing to distinguish intact RNAs from fragmented RNAs, reporting thousands of intact sperm RNA species conserved in mouse and human sperms.

Published in Nature Communications

ISSN: 2041-1723 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science
Website: https://www.nature.com/ncomms/

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