BioTechniques (Feb 2004)

Comparison of different probe-level analysis techniques for oligonucleotide microarrays

  • Barbara Rosati,
  • Frederic Grau,
  • Anneke Kuehler,
  • Samantha Rodriguez,
  • David McKinnon

DOI
https://doi.org/10.2144/04362mt03
Journal volume & issue
Vol. 36, no. 2
pp. 316 – 322

Abstract

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Three different software packages for the probe-level analysis of high-density oligonucleotide microarray data were compared using an experiment-derived data set that was validated using real-time PCR. The efficiency with which these three programs could identify true positives in this data set was assessed. In addition, estimates of false-positive and false-negative rates were determined. The performance of the programs using very small data sets was also compared, and recommendations for use are suggested.