Ovitrap surveillance of dengue vector mosquitoes in Bandung City, West Java Province, Indonesia

Abstract

Larval surveillance is the central approach for monitoring dengue vector populations in Indonesia. However, traditional larval indices are ineffective for measuring mosquito population dynamics and predicting the dengue transmission risk. We conducted a 14-month ovitrap surveillance. Eggs and immature mosquitoes were collected on a weekly basis from an urban village of Bandung, namely Sekejati. Ovitrap-related indices, namely positive house index (PHI), ovitrap index (OI), and ovitrap density index (ODI), were generated and correlated with environmental variables, housing type (terraced or high-density housing), ovitrap placement location (indoor or outdoor; household or public place), and local dengue cases. Our results demonstrated that Aedes aegypti was significantly predominant compared with Aedes albopictus at each housing type and ovitrap placement location. Ovitrap placement locations and rainfall were the major factors contributing to variations in PHI, OI, and ODI, whereas the influences of housing type and temperature were subtle. Indoor site values were significantly positively correlated to outdoor sites’ values for both OI and ODI. OI and ODI values from households were best predicted with those from public places at 1- and 0-week lags, respectively. Weekly rainfall values at 4- and 3-week lags were the best predictors of OI and ODI for households and public places, respectively. Monthly mean PHI, OI, and ODI were significantly associated with local dengue cases. In conclusion, ovitrap may be an effective tool for monitoring the population dynamics of Aedes mosquitoes, predicting dengue outbreaks, and serving as an early indicator to initiate environmental clean-up. Ovitrap surveillance is easy for surveyors if they are tasked with a certain number of ovitraps at a designated area, unlike the existing larval surveillance methodology, which entails identifying potential breeding sites largely at the surveyors’ discretion. Ovitrap surveillance may reduce the influence of individual effort in larval surveillance that likely causes inconsistency in results. Author summary The dengue virus, transmitted by Aedes vectors, has been continuously spreading in tropical and subtropical countries, causing illness and fatality. Given the lack of a cost-effective dengue vaccine, the vector control approach for reducing the Aedes population remains the key method for mitigating dengue transmission. For a successful vector control program, an effective vector surveillance system is crucial for precisely predicting the spatial and temporal risk of a dengue outbreak. The ovitrap system improves data collection efficiency, aiding long-term dengue vector monitoring activities. This study is one of the few long-term dengue vector surveillance programs in Indonesia and provides compelling evidence of the need to improve the existing conventional larval surveillance system. The results demonstrated that two dengue vector mosquitoes, A. aegypti and A. albopictus, were present in the study area, and A. aegypti was more prevalent than A. albopictus. We observed an interactive relationship between ovitrap placement and rainfall in the dynamics of ovitrap-related indices; understanding this relationship allows for timely initiation of vector control and intervention strategies. We conclude that the ovitrap surveillance system is a sensitive tool for monitoring the population dynamics of Aedes vectors, predicting dengue outbreaks, and potentially improving community-based conventional larval surveillance.