Nature Communications (Nov 2020)
CRISPR based editing of SIV proviral DNA in ART treated non-human primates
- Pietro Mancuso,
- Chen Chen,
- Rafal Kaminski,
- Jennifer Gordon,
- Shuren Liao,
- Jake A. Robinson,
- Mandy D. Smith,
- Hong Liu,
- Ilker K. Sariyer,
- Rahsan Sariyer,
- Tiffany A. Peterson,
- Martina Donadoni,
- Jaclyn B. Williams,
- Summer Siddiqui,
- Bruce A. Bunnell,
- Binhua Ling,
- Andrew G. MacLean,
- Tricia H. Burdo,
- Kamel Khalili
Affiliations
- Pietro Mancuso
- Department of Neuroscience, Center for Neurovirology, Lewis Katz School of Medicine at Temple University
- Chen Chen
- Department of Neuroscience, Center for Neurovirology, Lewis Katz School of Medicine at Temple University
- Rafal Kaminski
- Department of Neuroscience, Center for Neurovirology, Lewis Katz School of Medicine at Temple University
- Jennifer Gordon
- Department of Neuroscience, Center for Neurovirology, Lewis Katz School of Medicine at Temple University
- Shuren Liao
- Department of Neuroscience, Center for Neurovirology, Lewis Katz School of Medicine at Temple University
- Jake A. Robinson
- Department of Neuroscience, Center for Neurovirology, Lewis Katz School of Medicine at Temple University
- Mandy D. Smith
- Department of Neuroscience, Center for Neurovirology, Lewis Katz School of Medicine at Temple University
- Hong Liu
- Department of Neuroscience, Center for Neurovirology, Lewis Katz School of Medicine at Temple University
- Ilker K. Sariyer
- Department of Neuroscience, Center for Neurovirology, Lewis Katz School of Medicine at Temple University
- Rahsan Sariyer
- Department of Neuroscience, Center for Neurovirology, Lewis Katz School of Medicine at Temple University
- Tiffany A. Peterson
- Division of Comparative Pathology, Tulane National Primate Research Center
- Martina Donadoni
- Department of Neuroscience, Center for Neurovirology, Lewis Katz School of Medicine at Temple University
- Jaclyn B. Williams
- Division of Comparative Pathology, Tulane National Primate Research Center
- Summer Siddiqui
- Division of Comparative Pathology, Tulane National Primate Research Center
- Bruce A. Bunnell
- Division of Comparative Pathology, Tulane National Primate Research Center
- Binhua Ling
- Division of Comparative Pathology, Tulane National Primate Research Center
- Andrew G. MacLean
- Division of Comparative Pathology, Tulane National Primate Research Center
- Tricia H. Burdo
- Department of Neuroscience, Center for Neurovirology, Lewis Katz School of Medicine at Temple University
- Kamel Khalili
- Department of Neuroscience, Center for Neurovirology, Lewis Katz School of Medicine at Temple University
- DOI
- https://doi.org/10.1038/s41467-020-19821-7
- Journal volume & issue
-
Vol. 11,
no. 1
pp. 1 – 11
Abstract
Removal of integrated HIV DNA remains a roadblock for HIV cure. Here, Mancuso et al. show that intravenous administration of an adeno-associated virus-based CRISPR/Cas9 gene editing construct to SIV-infected macaques results in excision of integrated proviral DNA from infected blood cells and tissues known to be viral reservoirs.
