Proliferative and differentiated potential of mesenchymal stem cells from adipose tissue under cultivation conditions

Abstract

The purpose. Cultivation of mesenchymal stem cells (MSCs) from adipose tissue (AT) of experimental animals and humans to study induction of neurogenic differentiation.Materials and methods. AT samples from 30 experimental animals and 5 patients were used. MSCs cultures were studied for 5 passages. To induce neurogenic differentiation retinoic acid (1 mkM) and/or 5-azacytidine (1 mkM) were used. Cultures were observed in vivo, also according to cytological and immunohistochemical studies.Results. Fibroblast-like MSCs growth was observed in cell cultures from AT. At presence of specific differentiation factors they acquired neuron-like or astrocyte-like phenotype with positive staining to GFAP.Conclusions. In cultures of animal and human AT cells fraction of MSCs was derived, capable to induce neurogenic differentiating under the influence of retinoic acid and/or 5-azacytidine, as confirmed by immunohistochemistry.