Frontiers in Genetics (Oct 2013)

A comprehensive promoter landscape identifies a novel promoter for CD133 in restricted tissues, cancers and stem cells

  • Ramakrishna eSompallae,
  • Oliver Marc Hofmann,
  • Oliver Marc Hofmann,
  • Christopher A Maher,
  • Craig eGedye,
  • Andreas eBehren,
  • Morana eVitezic,
  • Morana eVitezic,
  • Carsten O Daub,
  • Carsten O Daub,
  • Sylvie eDevalle,
  • Otavia L Caballero,
  • Piero eCarninci,
  • Yoshihide eHayashizaki,
  • Elizabeth R Lawlor,
  • Jonathan eCebon,
  • Winston eHide,
  • Winston eHide

DOI
https://doi.org/10.3389/fgene.2013.00209
Journal volume & issue
Vol. 4

Abstract

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PROM1 is the gene encoding prominin-1 or CD133, an important cell surface marker for the isolation of both normal and cancer stem cells. PROM1 transcripts initiate at a range of transcription start sites (TSS) associated with distinct tissue and cancer expression profiles. Using high resolution Cap Analysis of Gene Expression (CAGE) sequencing we characterize TSS utilization across a broad range of normal and developmental tissues. We identify a novel proximal promoter (P6) within CD133+ melanoma cell lines and stem cells. Additional exon array sampling finds P6 to be active in populations enriched for mesenchyme, neural stem cells and within CD133+ enriched Ewing sarcomas. The P6 promoter is enriched with respect to previously characterized PROM1 promoters for a HMGI/Y (HMGA1) family transcription factor binding site motif and exhibits different epigenetic modifications relative to the canonical promoter region of PROM1.

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