The Effect of Methyl-Derivatives of Flavanone on MCP-1, MIP-1β, RANTES, and Eotaxin Release by Activated RAW264.7 Macrophages
Małgorzata Kłósek,
Anna Kurek-Górecka,
Radosław Balwierz,
Agnieszka Krawczyk-Łebek,
Edyta Kostrzewa-Susłow,
Joanna Bronikowska,
Dagmara Jaworska,
Zenon P. Czuba
Affiliations
Małgorzata Kłósek
Department of Microbiology and Immunology, Faculty of Medical Sciences, Medical University of Silesia in Katowice, Jordana 19, 41-808 Zabrze, Poland
Anna Kurek-Górecka
Department of Community Pharmacy, Faculty of Pharmaceutical Sciences in Sosnowiec, Medical University of Silesia in Katowice, St Kasztanowa 3, 41-200 Sosnowiec, Poland
Radosław Balwierz
Institute of Chemistry, University of Opole, Oleska 48, 45-052 Opole, Poland
Agnieszka Krawczyk-Łebek
Department of Food Chemistry and Biocatalysis, University of Environmental and Life Sciences, Norwida 25, 50-375 Wrocław, Poland
Edyta Kostrzewa-Susłow
Department of Food Chemistry and Biocatalysis, University of Environmental and Life Sciences, Norwida 25, 50-375 Wrocław, Poland
Joanna Bronikowska
Department of Microbiology and Immunology, Faculty of Medical Sciences, Medical University of Silesia in Katowice, Jordana 19, 41-808 Zabrze, Poland
Dagmara Jaworska
Department of Microbiology and Immunology, Faculty of Medical Sciences, Medical University of Silesia in Katowice, Jordana 19, 41-808 Zabrze, Poland
Zenon P. Czuba
Department of Microbiology and Immunology, Faculty of Medical Sciences, Medical University of Silesia in Katowice, Jordana 19, 41-808 Zabrze, Poland
Chemokines, also known as chemotactic cytokines, stimulate the migration of immune cells. These molecules play a key role in the pathogenesis of inflammation leading to atherosclerosis, neurodegenerative disorders, rheumatoid arthritis, insulin-resistant diabetes, and cancer. Moreover, they take part in inflammatory bowel disease (IBD). The main objective of our research was to determine the activity of methyl-derivatives of flavanone, namely, 2′-methylflavanone (5B), 3′-methylflavanone (6B), 4′-methylflavanone (7B), and 6-methylflavanone (8B), on the releasing of selected cytokines by RAW264.7 macrophages activated by LPS. We determined the concentration of chemokines belonging to the CC chemokine family, namely, MCP-1, MIP-1β, RANTES, and eotaxin, using the Bio-Plex Magnetic Luminex Assay and the Bio-PlexTM 200 System. Among the tested compounds, only 5B and 6B had the strongest effect on inhibiting the examined chemokines’ release by macrophages. Therefore, 5B and 6B appear to be potentially useful in the prevention of diseases associated with the inflammatory process.
