Time lapse recording of cortical interneuron migration in mouse organotypic brain slices and explants

Abstract

Summary: Interneuron migration involves repetitive cycles of pausing and motion that include nucleokinesis and dynamic branching of the leading process. Here, we provide a step-by-step description of how to culture and record the migration of cortical interneurons. We provide two culture models: the first includes organotypic brain slices and the second medial ganglionic eminence (MGE) explants. While organotypic brain slices provide a close-to-physiological context to analyze interneuron migration into cortical streams, MGE explants are appropriate to investigate the fine details of interneuron morphology remodeling during movement.For complete details on the use and execution of this protocol, please refer to Silva et al. (2018).

Keywords

• Cell Biology
• Microbiology
• Neuroscience