.Study of adsorption of factor vVIIIVFW complex on ion-exchange supports.

Abstract

We investigated the binding capacity for FVIII/vWF complex of five different anion-exchange supports, DEAE-Toyopearl 650M, Toyopearl Super Q-650M, Fractogel EMD TMAE (M), DEAE Sepharose FF, Fractogel EMD TMAE Hicap (M). The chromatographic supports with tentacle structure demonstrated the best results. The adsorption was performed by a batch-method when mixing at the room t0 and a residual FVIII and vWF-content was measured in the supernatant. The maximum binding capacity was estimated 188 IU FVIII /g and 2309 IU vWF /g for Fractogel EMD TMAE (M) and 134 IU FVIII /g and 1935 IU vWF /g for Fractogel EMD TMAE Hicap (M). The results obtained may be used for development of the method of purifying a FVIII/vWF complex using ion-exchange chromatography.

Keywords

• coagulation blood factors, sorbents for chromatography, cryoprecipitate