Evaluation of the Inhibitory Potential of Casuarictin, an Ellagitannin Isolated from White Mangrove (<i>Laguncularia racemosa</i>) Leaves, on Snake Venom Secretory Phospholipase A2
Caroline Fabri Bittencourt Rodrigues,
Marcelo José Pena Ferreira,
Mariana Novo Belchor,
Caroline R. C. Costa,
Danielle P. Novaes,
Adeilso Bispo dos Santos Junior,
Cinthia I. Tamayose,
Marcus Vinícius Terashima Pinho,
Marcos Antonio de Oliveira,
Marcos Hikari Toyama
Affiliations
Caroline Fabri Bittencourt Rodrigues
Laboratório de Bioquímica e Biologia Molecular de Peptídeos (BIOMOLPEP), Instituto de Biociências, UNESP, Campus do Litoral Paulista, São Vicente 11330-900, São Paulo, Brazil
Marcelo José Pena Ferreira
Departamento de Botânica, Instituto de Biociências, Universidade de São Paulo, São Paulo 05508-090, Brazil
Mariana Novo Belchor
Laboratório de Bioquímica e Biologia Molecular de Peptídeos (BIOMOLPEP), Instituto de Biociências, UNESP, Campus do Litoral Paulista, São Vicente 11330-900, São Paulo, Brazil
Caroline R. C. Costa
Laboratório de Bioquímica e Biologia Molecular de Peptídeos (BIOMOLPEP), Instituto de Biociências, UNESP, Campus do Litoral Paulista, São Vicente 11330-900, São Paulo, Brazil
Danielle P. Novaes
Laboratório de Bioquímica e Biologia Molecular de Peptídeos (BIOMOLPEP), Instituto de Biociências, UNESP, Campus do Litoral Paulista, São Vicente 11330-900, São Paulo, Brazil
Adeilso Bispo dos Santos Junior
Laboratório de Bioquímica e Biologia Molecular de Peptídeos (BIOMOLPEP), Instituto de Biociências, UNESP, Campus do Litoral Paulista, São Vicente 11330-900, São Paulo, Brazil
Cinthia I. Tamayose
Departamento de Botânica, Instituto de Biociências, Universidade de São Paulo, São Paulo 05508-090, Brazil
Marcus Vinícius Terashima Pinho
Laboratório de Bioquímica e Biologia Molecular de Peptídeos (BIOMOLPEP), Instituto de Biociências, UNESP, Campus do Litoral Paulista, São Vicente 11330-900, São Paulo, Brazil
Marcos Antonio de Oliveira
Laboratório de Biologia Molecular Estrutural (LABIMES), Instituto de Biociências, UNESP, Campus do Litoral Paulista, São Vicente 11330-900, São Paulo, Brazil
Marcos Hikari Toyama
Laboratório de Bioquímica e Biologia Molecular de Peptídeos (BIOMOLPEP), Instituto de Biociências, UNESP, Campus do Litoral Paulista, São Vicente 11330-900, São Paulo, Brazil
Ellagitannins constitute the largest group of hydrolyzable tannins of plants, and, from this group, casuarictin (Casu) was identified in some plant species. However, to our knowledge, no investigation of secretory phospholipase A2 (sPLA2) inhibition by Casu has been performed yet. Casuarictin was isolated by chromatography n-butanol (n-BuOH) partition of Laguncularia racemosa leaves. The pharmacological and biological effects of Casu were evaluated on isolated sPLA2 from the rattlesnake (Crotalus durissus terrificus) and using a plant bacterial strain. The compound was able to form a protein complex consisting of a stable sPLA2 + Casu complex. Analyses carried out with matrix-assisted laser desorption ionization-time-of-flight mass spectrometry (MALDI-TOF) revealed that the molecular mass of sPLA2 increased from 14,425.62 to 15,362.74 Da. The enzymatic activity of the sPLA2 + Casu complex was significantly lower than that of native sPLA2. Besides, molecular interactions of Casu with sPLA2 were able to virtually abolish the native edematogenic effect as well as myonecrosis induced by the protein when injected 10 min after sPLA2. Therefore, Casu may be considered a potential anti-inflammatory that can be used to treat edema and myonecrosis induced by serine-secreting phospholipase A2. In addition, the compound also showed great antimicrobial potential.
