Multiple sgRNAs facilitate base editing-mediated i-stop to induce complete and precise gene disruption

• Kun Jia,
• Zongyang Lu,
• Fei Zhou,
• Zhiqi Xiong,
• Rui Zhang,
• Zhiwei Liu,
• Yu’e Ma,
• Lei He,
• Cong Li,
• Zhen Zhu,
• Dejing Pan,
• Zhengxing Lian

Affiliations

Kun Jia

Beijing Key Laboratory of Animal Genetic Improvement, China Agricultural University

Zongyang Lu

School of Life Science and Technology, ShanghaiTech University

Fei Zhou

Cambridge-Suda Genomic Resource Center and Jiangsu Key Laboratory of Neuropsychiatric Diseases Research, Soochow University

Zhiqi Xiong

School of Life Science and Technology, ShanghaiTech University

Rui Zhang

Beijing Key Laboratory of Animal Genetic Improvement, China Agricultural University

Zhiwei Liu

Cambridge-Suda Genomic Resource Center and Jiangsu Key Laboratory of Neuropsychiatric Diseases Research, Soochow University

Yu’e Ma

Cambridge-Suda Genomic Resource Center and Jiangsu Key Laboratory of Neuropsychiatric Diseases Research, Soochow University

Lei He

Cambridge-Suda Genomic Resource Center and Jiangsu Key Laboratory of Neuropsychiatric Diseases Research, Soochow University

Cong Li

Cambridge-Suda Genomic Resource Center and Jiangsu Key Laboratory of Neuropsychiatric Diseases Research, Soochow University

Zhen Zhu

Key Laboratory of MEMS of Ministry of Education, Southeast University

Dejing Pan

Cambridge-Suda Genomic Resource Center and Jiangsu Key Laboratory of Neuropsychiatric Diseases Research, Soochow University

Zhengxing Lian

Beijing Key Laboratory of Animal Genetic Improvement, China Agricultural University