Nature Communications (Mar 2019)

Synthetic energy sensor AMPfret deciphers adenylate-dependent AMPK activation mechanism

  • Martin Pelosse,
  • Cécile Cottet-Rousselle,
  • Cécile M. Bidan,
  • Aurélie Dupont,
  • Kapil Gupta,
  • Imre Berger,
  • Uwe Schlattner

DOI
https://doi.org/10.1038/s41467-019-08938-z
Journal volume & issue
Vol. 10, no. 1
pp. 1 – 13

Abstract

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AMP-activated protein kinase AMPK senses and regulates cellular energy state. Here the authors engineer a synthetic sensor, AMPfret, that allows direct, real-time readout of the AMPK conformational state by fluorescence resonance energy transfer (FRET).