Activation of long-non-coding RNA NEAT1 sponging microRNA-147 inhibits radiation damage by targeting PDPK1 in troxerutin radioprotection
Yong-jian Hu,
Gui-yuan Song,
Fan Zhang,
Nan Zhang,
Fei Wang,
Jing-long Wang,
Xia Wang,
Tao-yang Wang,
Yu-feng Li,
Yi-di Yan,
Wen-tao Dou,
Chen-yi Cheng,
Ping Xu
Affiliations
Yong-jian Hu
School of Food and Biomedicine, Zaozhuang University, Zaozhuang, Shandong 277160, China; Henan Key Laboratory of Medical Tissue Regeneration, Xinxiang Medical University, Xinxiang, Henan 453003, China
Gui-yuan Song
School of Food and Biomedicine, Zaozhuang University, Zaozhuang, Shandong 277160, China; School of Public Health, Weifang Medical University, Weifang, Shandong 261000, China; Radiology Laboratory, Central Laboratory, Rizhao People’s Hospital, Rizhao, Shandong 276800, China
Fan Zhang
School of Food and Biomedicine, Zaozhuang University, Zaozhuang, Shandong 277160, China; Henan Key Laboratory of Medical Tissue Regeneration, Xinxiang Medical University, Xinxiang, Henan 453003, China
Nan Zhang
School of Food and Biomedicine, Zaozhuang University, Zaozhuang, Shandong 277160, China
Fei Wang
School of Food and Biomedicine, Zaozhuang University, Zaozhuang, Shandong 277160, China
Jing-long Wang
School of Food and Biomedicine, Zaozhuang University, Zaozhuang, Shandong 277160, China
Xia Wang
College of Medical Laboratory, Xinxiang Medical University, Xinxiang, Henan 453003, China
Tao-yang Wang
School of Food and Biomedicine, Zaozhuang University, Zaozhuang, Shandong 277160, China; Henan Key Laboratory of Medical Tissue Regeneration, Xinxiang Medical University, Xinxiang, Henan 453003, China
Yu-feng Li
Radiology Laboratory, Central Laboratory, Rizhao People’s Hospital, Rizhao, Shandong 276800, China
Yi-di Yan
Basic Medical School, Xinxiang Medical University, Xinxiang, Henan 453003, China
Wen-tao Dou
Basic Medical School, Xinxiang Medical University, Xinxiang, Henan 453003, China
Chen-yi Cheng
Basic Medical School, Xinxiang Medical University, Xinxiang, Henan 453003, China
Ping Xu
School of Food and Biomedicine, Zaozhuang University, Zaozhuang, Shandong 277160, China; Henan Key Laboratory of Medical Tissue Regeneration, Xinxiang Medical University, Xinxiang, Henan 453003, China; Corresponding author
Summary: A better understanding of the molecular mechanism involving the lncRNA-miRNA-mRNA network underlying radiation damage can be beneficial for radioprotection. This study was designed to investigate the potential role of lncRNA NEAT1, miR-147 and Phosphoinositide Dependent Protein Kinase 1 (PDPK1) interaction in radioprotection by troxerutin (TRT). We first demonstrated that NEAT1 sponged miR-147, and PDPK1 mRNA was the primary target of miR-147. In the cells, the NEAT1 and PDPK1 levels were downregulated after the radiation but increased after the treatment with TRT. The miR-147 level was significantly induced by radiation and inhibited by TRT. NEAT1 negatively regulated the expression of miR-147, whereas miR-47 targeted PDPK1 to downregulate its expression. In radioprotection, TRT effectively upregulated NEAT1 to inhibit miR-147 and to upregulate PDPK1. We concluded that TRT could promote radioprotection by stimulating NEAT1 to upregulate PDPK1 expression by suppressing miR-147. NEAT1 could be a critical therapeutic target of radiation damage.
