Whole chromosome loss and genomic instability in mouse embryos after CRISPR-Cas9 genome editing

Stamatis Papathanasiou; Styliani Markoulaki; Logan J. Blaine; Mitchell L. Leibowitz; Cheng-Zhong Zhang; Rudolf Jaenisch; David Pellman

doi:10.1038/s41467-021-26097-y

Nature Communications (Oct 2021)

Whole chromosome loss and genomic instability in mouse embryos after CRISPR-Cas9 genome editing

Stamatis Papathanasiou,
Styliani Markoulaki,
Logan J. Blaine,
Mitchell L. Leibowitz,
Cheng-Zhong Zhang,
Rudolf Jaenisch,
David Pellman

Affiliations

Stamatis Papathanasiou: Department of Cell Biology, Blavatnik Institute, Harvard Medical School
Styliani Markoulaki: Whitehead Institute
Logan J. Blaine: Department of Cell Biology, Blavatnik Institute, Harvard Medical School
Mitchell L. Leibowitz: Department of Cell Biology, Blavatnik Institute, Harvard Medical School
Cheng-Zhong Zhang: Department of Biomedical Informatics, Harvard Medical School
Rudolf Jaenisch: Whitehead Institute
David Pellman: Department of Cell Biology, Blavatnik Institute, Harvard Medical School

DOI: https://doi.org/10.1038/s41467-021-26097-y
Journal volume & issue: Vol. 12, no. 1
pp. 1 – 7

Abstract

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A possible undesired outcome of CRISPR-Cas9 germline editing is unwanted karyotype alterations. Here the authors track aberrations through three divisions of embryonic development following Cas9 editing.

Published in Nature Communications

ISSN: 2041-1723 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science
Website: https://www.nature.com/ncomms/

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